We narrowed to 2,848 results for: lox

1. pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP

   Plasmid

   #188774

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP

   Plasmid

   #192822

   Purpose

   Golden Gate compatible plasmid with mCherry and Neo

   Depositor

   Mark Denham

   Article

   Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

   Insert

   mCherry and Neo
   

   Expression

   Mammalian

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

   Plasmid

   #89587

   Purpose

   For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nuclear localization signal (nls) turboRFP
   

   Expression

   Mammalian

   Available Since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2ADW-lox-tdKeima-NES-lox-6011nls(ERK)-P2Av3-RGECO1

   Plasmid

   #162486

   Purpose

   For generating transgenic mice expressing ERK biosensor and RGECO1

   Depositor

   Michiyuki Matsuda

   Article

   Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

   Insert

   ERK biosensor based on FRET, and RGECO1
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

   Plasmid

   #175259

   Purpose

   Dual plasmid labeling membrane target protein of different neuronal population with different fluorophore

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   Lox-rox-STOP-rox-lyn-mScarlet-pA-lox
   

   Use

   Cre/Lox; Dre/rox

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   pCAG

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272

   Plasmid

   #154829

   Purpose

   Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   coEPO
   coEPO
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   Codon-optimized (Cricetulus griseus)

   Promoter

   100RPU.2

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

   Plasmid

   #129538

   Purpose

   modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

   Depositor

   Arneet Saltzman

   Article

   Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox; Mos1-mediated single copy insertion (mos…

   Expression

   Worm

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

   Plasmid

   #224522

   Purpose

   A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
   

   Use

   Cre/Lox

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD

   Plasmid

   #196878

   Purpose

   Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-N-gTBD
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1

    Plasmid

    #196879

    Purpose

    Neuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD

    Plasmid

    #196894

    Purpose

    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    N-gTBD-mOrange2 (NEDD1 Human)
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196881

    Purpose

    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD
    

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox

    Plasmid

    #168770

    Purpose

    B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Id1 (Id1 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting; Dre / rox

    Mutation

    See Depositor Comments Below

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC18 Apln5-HA-FlpO-pA-LoxP-PGK-Neo-pA-LoxP-Apln3 (SO89)

    Plasmid

    #159222

    Purpose

    Can be used to target the mouse Apln locus in mouse eggs or ES cells, in order to drive the mosaic expression of the protein HA-NLS-FlpO

    Depositor

    Rui Benedito

    Article

    Luo et al Nature. 2021 Jan;589(7842):437-441. doi:

    Insert

    Apln 5'-FlpO-WPRE-Sv40pA-LoxP-PGK-Neo-pA-LoxP-Apln
    

    Expression

    Mammalian

    Promoter

    Apln

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85006

    Purpose

    For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:

    Plasmid

    #179585

    Purpose

    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    NLS-NG-T2A-hSHH
    

    Use

    Piggybac

    Tags

    NLS-NG-T2A

    Promoter

    CAGp

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #69138

    Purpose

    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

    Depositor

    Takuji Iwasato

    Article

    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

    Insert

    CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
    

    Tags

    none

    Expression

    Mammalian

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

    Plasmid

    #179586

    Purpose

    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

    Depositor

    Ali Hemmati Brivanlou

    Article

    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

    Insert

    NLS-NG-T2A-hSHH
    

    Use

    Piggybac

    Tags

    NeonGreen

    Promoter

    TRE

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85179

    Purpose

    For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nuclear localization signal (nls) tagRFP
    

    Expression

    Mammalian

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

    Plasmid

    #217509

    Purpose

    KD Recombinase dependent conditional 1X ALFA cassette

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA
    

    Tags

    ALFA tag

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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