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Showing: 2761 - 2800 of 3064 results

  1. pKDR-EF1a.13

    Plasmid
    #183576
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 13
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183576-plasmid-map-sequence-id-370309

    Enlarge

  2. pKDR-TetOn.7

    Plasmid
    #183581
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 7
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183581-plasmid-map-sequence-id-365496

    Enlarge

  3. pKDR-TetOn.5

    Plasmid
    #183580
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183580-plasmid-map-sequence-id-365495

    Enlarge

  4. pKDR-TetOn.9

    Plasmid
    #183582
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183582-plasmid-map-sequence-id-365494

    Enlarge

  5. pKDR-TetOn.11

    Plasmid
    #183583
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 11
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183583-plasmid-map-sequence-id-365493

    Enlarge

  6. pKDR-CBh.6

    Plasmid
    #183559
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 6
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183559-plasmid-map-sequence-id-365458

    Enlarge

  7. pKDR-CBh.8

    Plasmid
    #183560
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 8
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183560-plasmid-map-sequence-id-365456

    Enlarge

  8. pKDR-CBh.13

    Plasmid
    #183562
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 13
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183562-plasmid-map-sequence-id-365455

    Enlarge

  9. pKDR-CBh.4

    Plasmid
    #183558
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183558-plasmid-map-sequence-id-365454

    Enlarge

  11. pKDR-CBh.18

    Plasmid
    #183564
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 18
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183564-plasmid-map-sequence-id-365415

    Enlarge

  12. pKDR-CBh.10

    Plasmid
    #183561
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 10
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183561-plasmid-map-sequence-id-365414

    Enlarge

  15. pKDR-TetOn.13

    Plasmid
    #183584
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 13
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183584-plasmid-map-sequence-id-365382

    Enlarge

  16. pKDR-EF1a.10

    Plasmid
    #183573
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 10
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183573-plasmid-map-sequence-id-365381

    Enlarge

  17. pKDR-EF1a.11

    Plasmid
    #183574
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 11
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183574-plasmid-map-sequence-id-365378

    Enlarge

  18. pKDR-EF1a.12

    Plasmid
    #183575
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 12
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183575-plasmid-map-sequence-id-365377

    Enlarge

  19. pKDR-EF1a.5

    Plasmid
    #183572
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183572-plasmid-map-sequence-id-365373

    Enlarge

  20. pKDR-EF1a.3

    Plasmid
    #183570
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183570-plasmid-map-sequence-id-365366

    Enlarge

  21. pKDR-EF1a.1

    Plasmid
    #183568
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183568-plasmid-map-sequence-id-365365

    Enlarge

  22. pKDR-CBh.21

    Plasmid
    #183565
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)
    Depositor
    Erik Dent
    Article
    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d
    Insert
    Attenuator Sequence 21
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    June 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    183565-plasmid-map-sequence-id-364046

    Enlarge

  23. pMRBad-C-DiB-RM

    Plasmid
    #168478
    Purpose
    C-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    C-fragment of DiB-RM
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168478-plasmid-map-sequence-id-332738

    Enlarge

  24. pET11a-N-DiB-RM

    Plasmid
    #168476
    Purpose
    N-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    N-fragment of DiB-RM
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168476-plasmid-map-sequence-id-332734

    Enlarge

  25. pET11a-Z-N-DiB2

    Plasmid
    #168473
    Purpose
    N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10
    Insert
    N-fragment of DiB2 + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168473-plasmid-map-sequence-id-332731

    Enlarge

  26. pET11a-Z-N-wtBlc

    Plasmid
    #168258
    Purpose
    N-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:
    Insert
    N-fragment of wtBlc + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168258-plasmid-map-sequence-id-332729

    Enlarge

  33. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

    Enlarge

  34. ampl43

    Plasmid
    #161830
    Purpose
    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.
    Depositor
    Saeed Tavazoie
    Article
    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    161830-plasmid-map-sequence-id-318315

    Enlarge

  35. BPK1520

    Plasmid
    #65777
    Purpose
    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SpCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65777-plasmid-map-sequence-id-316938

    Enlarge

  36. pYPQ220-Ultra

    Plasmid
    #176889
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    AsCas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176889-plasmid-map-sequence-id-350058

    Enlarge

  39. pYPQ230-E795L

    Plasmid
    #176890
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    LbCas12a-E795L
    Use
    CRISPR; Gateway compatible lbcpf1 entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176890-plasmid-map-sequence-id-354003

    Enlarge

  40. pLP-pTac-Tn5-chlorR

    Plasmid
    #199994
    Purpose
    Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.
    Depositor
    Farren Isaacs
    Article
    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.
    Insert
    Landing Pads
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    199994-plasmid-map-sequence-id-423257

    Enlarge

Showing: 2761 - 2800 of 3064 results