We narrowed to 4,751 results for: MLS

1. pMKO.1-shCdkn3

   Plasmid

   #160955

   Purpose

   Cdkn3 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Cdkn3 shRNA (Cdkn3 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMKO.1-shCenpf

   Plasmid

   #160956

   Purpose

   Cenpf shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Cenpf shRNA (Cenpf Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-EGxxFP-CD55

   Plasmid

   #153959

   Purpose

   Used for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequences

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   CD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
   

   Use

   A plasmid specific for egxxfp assay

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CAG promoter

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. prCOrev

   Plasmid

   #153961

   Purpose

   Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
   

   Use

   Retroviral; A reporter plasmid detecting dna reco…

   Tags

   NLS-ZF

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   LTR

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. prCO

   Plasmid

   #153960

   Purpose

   Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
   

   Use

   Retroviral; A reporter plasmid detecting dna reco…

   Tags

   NLS-ZF

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   LTR

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER

   Plasmid

   #123354

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   cp-moxCerulean3-moxCerulean3-FLARE-D1ER
   

   Use

   Tags

   C-terminal ER retention signal (KDEL), cp-moxCeru…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

   Plasmid

   #123340

   Purpose

   Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-EKAR-EV(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-mCherry-mCherry-FLARE-CKAR

   Plasmid

   #123345

   Purpose

   Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   mCherry-mCherry-FLARE-CKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

   Plasmid

   #123344

   Purpose

   Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-CKAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

    Plasmid

    #123336

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-cpCerulean3-FLARE-AKAR
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR

    Plasmid

    #123335

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-AKAR
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRC/CMV-TYK2VF/PA-VSV

    Plasmid

    #139351

    Purpose

    Expression of the double mutant of TYK2-V678F-P1104A

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-V678F-P1104A

    Promoter

    CMV

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. INCTbiosyn-pUB-HspINT2

    Plasmid

    #127512

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 2.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 2 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. INCTbiosyn-pUB-HspINT4

    Plasmid

    #127513

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 4.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 4 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. INCTbiosyn-pUB-HspINT5

    Plasmid

    #127514

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 5.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 5 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. INCTbiosyn-pUB-HspINT9

    Plasmid

    #127516

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 9.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 9 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. INCTbiosyn-pUB-HspINT13

    Plasmid

    #127517

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 13.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 13 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. [pSK481] pLJC5 Flag-Depdc5(B)

    Plasmid

    #109339

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant B

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    EQPLH(371-375)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. [pSK479] pLJC5 Flag-Depdc5(C)

    Plasmid

    #109340

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant C

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    FTPRI(419-423)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWZLblasti-HA-MEK5 CBM-E

    Plasmid

    #53169

    Purpose

    Expresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L280A/H281A/M323A/Q332A

    Promoter

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only