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We narrowed to 98 results for: MLS

Showing: 1 - 20 of 98 results

  1. Common Injection Routes in Mice

    Type
    Blog Post
    ...27 < 2–3 mL (~1 mL per site) Beginner Intradermal Anesthesia Between skin layers 26 < 0.05 mL Advanced... back or hind legs Gauge: 25–27 Volume: < 2–3 ml (~1 ml per site) Skill required: beginner Intradermal...–27 < 2–3 mL Intermediate Intramuscular Mechanical restrainer Thigh 25–27 < 0.02–0.05 mL Beginner ...cord 30 up to 0.01 mL Expert Intratracheal Anesthesia Trachea 20–23 (tubing) < 0.07 mL Advanced Intranasal...Nose n/a < 0.05 mL Beginner Oral gavage Scruffing Throat 18–20 (bulb tipped) up to 10 mL/kg Intermediate...vein or retro-orbitally Gauge: 27–30 Volume: < 0.2 ml Skill required: beginner (retro-orbital) to intermediate...Location: peritoneal cavity Gauge: 25–27 Volume: < 2–3 ml Skill required: intermediate Intramuscular Intramuscular...

  2. AAV Packaged on Request

    Type
    Collection
    ... µL aliquots 1 mL 4 × 10 12 GC/mL* 1 × 10 13 GC/mL* Medium 25 × 100 µL aliquots 2.5 mL Large 50 × 100 ...AAV6, AAV8, AAV11, or AAVrg) and volume (1 mL, 2.5 mL, or 5 mL). Currently, we can only package plasmids...µL aliquots 5 mL *AAV2 yields are generally lower, with a minimum titer of 2 × 10 12 GC/mL and a typical...typical titer of 7 × 10 12 GC/mL. For more details, see our Scope of Service . All-in-One Pricing The price...

  3. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...-100 ug/mL Ampicillin beta-lactam Inhibits cell wall synthesis Bactericidal 100-200 ug/mL Carbenicillin...Bactericidal 100 ug/mL Bleomycin glycopeptide Induces DNA breaks Bactericidal 5-100 ug/mL Erythromycin...subunit; causes mis-translation Bactericidal 50-100 ug/mL Spectinomycin aminoglycoside Binds 30S ribosomal...interrupts protein synthesis Bactericidal 7.5-50 ug/mL Streptomycin aminoglycoside Inhibits initiation...aminoacyl translocation Bacteriostatic 50-100 ug/mL in EtOH Polymyxin B polypeptide Alters outer ...outer membrane permeability Bactericidal 10-100 ug/mL Tetracycline tetracyclin Binds 30S ribosomal subunit...synthesis (elongation step) Bacteriostatic 10 ug/mL Chloramphenicol   Binds 50S ribosomal subunit; ...

  4. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...AAV Retrograde (AAVrg), and volumes of 1 mL, 2.5 mL, or 5 mL. Please note that all orders will be shipped...expect titers of 1 × 1013 GC/mL for AAV1, AAV5, AAV8, and AAVrg and 7 × 1012 GC/mL for AAV2. There are no quotes...

  5. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ... Inhibits termination step of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3, CHO, 293HEK, Jurkat...synthesis at 80S; inhibits chain elongation 100-800 ug/mL Hygromycin B hygB HeLa, NIH3T3, CHO, Jurkat T cells...synthesis at 80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK, Jurkat T cells Inhibits...protein synthesis; premature chain termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3, CHO, COS-1, 293HEK,...Complexes with DNA; causes strand scissions 100-400 ug/mL *Not comprehensive.   ** In eukaryotes.   ***The...

  6. Tips for arabidopsis transformation

    Type
    Blog Post
    ...transformant. I like to spin down 100 mL of culture and resuspend in 25 mL of transformation medium per Agrobacterium...density in 18-24 hours. I then add 0.5 mL of this starter culture to 100 mL fresh media, which is then ready...wide enough to dunk your plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are both great...stock or a colony from an LB plate. In my hands, a 5 mL starter culture inoculated from a colony on a plate...

  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...restriction enzyme (5,000 U/ml), 5.0 μl ATP (10 mM), 0.25 μl (5 μg) BSA (20 mg/ml), 0.375 μl (750 U) T4 DNA...and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated oligos may be ordered instead...DNA ligase(2,000,000 U/ml), and H 2 O to final volume of 50 μl. This reaction may be scaled down to a ...onto a lysogeny broth (LB) agar plate with 100 μg/ml ampicillin and incubate O/N at 37 °C. Pick 2 - 3 ...Immediately transfer solution from cuvette into 1 ml of culture media after electroporation. Minimize ...proliferated when the concentration exceeds ~100,000 cells/ml or for adherent cells, the cells have reached ~80%...

  8. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...will turn yellow. Do NOT change the media (add 2-3 mL of fresh media once a week), and do NOT harvest the...days) Physical titer (particle count, rAdV genomes/mL) can be measured by: Optical density: particle concentration... of at 260nm corresponds to 1.1 x 1012 particles/mL. To calculate the particle number: OD260 reading ... x 1.1 x 1012 particles = number of particles per mL of sample. The purity of the sample can be estimated...Forming Units (PFU) or Infectious Units (IU), per mL)  Plaque formation assay: permissive cells are infected...

  11. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...drying out) while 3 ml of melted agarose are mixed with 3 ml of 2X DMEM in a 50 ml sterile tube (for 60... 60 mm dishes only 1.5 ml of each are used). This solution is capped, swirled and tested for the correct...

  14. Viral Production

    Type
    Collection
    ... is ensured to be less than 5 endotoxin units per mL. The endotoxin levels are determined using a chromogenic...-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression...using a 293T cell line in the presence of 10.0 μg/mL polybrene. This ensures that any loss of titer associated...

  15. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ... a titer of 4 x 1012 genome copies/mL or 8 x 1012 genome copies/mL and both results would be valid. This...titers tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute your virus before adding...can then be used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies...

  17. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...nucleases for highly efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. 31(2):294-301. PubMed... for making customized zinc-finger arrays. Maeder ML et al. Nat Protoc. . 2009. Sept 17. 4(10):1471-1501...

  18. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...yield** Miniprep 1–5 mL 5–50 µg Midiprep 25–100 mL 20–200 µg Maxiprep 100–500 mL 100–750 µg Megaprep...for example. These kits are designed to handle ~1–5 mL of liquid culture. Using a higher volume can affect...
Showing: 1 - 20 of 98 results