Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 12 results for: MLS

Showing: 1 - 12 of 12 results

  1. AAV Packaged on Request

    Type
    Collection
    ... µL aliquots 1 mL 4 × 10 12 GC/mL* 1 × 10 13 GC/mL* Medium 25 × 100 µL aliquots 2.5 mL Large 50 × 100 ...AAV6, AAV8, AAV11, or AAVrg) and volume (1 mL, 2.5 mL, or 5 mL). Currently, we can only package plasmids...µL aliquots 5 mL *AAV2 yields are generally lower, with a minimum titer of 2 × 10 12 GC/mL and a typical...typical titer of 7 × 10 12 GC/mL. For more details, see our Scope of Service . All-in-One Pricing The price...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...restriction enzyme (5,000 U/ml), 5.0 μl ATP (10 mM), 0.25 μl (5 μg) BSA (20 mg/ml), 0.375 μl (750 U) T4 DNA...and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated oligos may be ordered instead...DNA ligase(2,000,000 U/ml), and H 2 O to final volume of 50 μl. This reaction may be scaled down to a ...onto a lysogeny broth (LB) agar plate with 100 μg/ml ampicillin and incubate O/N at 37 °C. Pick 2 - 3 ...Immediately transfer solution from cuvette into 1 ml of culture media after electroporation. Minimize ...proliferated when the concentration exceeds ~100,000 cells/ml or for adherent cells, the cells have reached ~80%...

  5. Viral Production

    Type
    Collection
    ... is ensured to be less than 5 endotoxin units per mL. The endotoxin levels are determined using a chromogenic...-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression...using a 293T cell line in the presence of 10.0 μg/mL polybrene. This ensures that any loss of titer associated...

  6. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...nucleases for highly efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. 31(2):294-301. PubMed... for making customized zinc-finger arrays. Maeder ML et al. Nat Protoc. . 2009. Sept 17. 4(10):1471-1501...

  7. Antibody Production

    Type
    Collection
    ...concentrating to a final concentration of 0.9–1.1 mg/mL. Antibody concentration is confirmed by Coomassie...

  8. p53 Pathway

    Type
    Collection
    ...CBP in p53-dependent signal pathways. Avantaggiati ML, Ogryzko V, Gardner K, Giordano A, Levine AS, Kelly...

  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ... IL-22, IL-27, IL27 IL17F interleukin 17F IL-17F, ML-1, ML1 IL17RA interleukin 17 receptor A CD217, CDw217...betaglycan THPO thrombopoietin MGC163194, MGDF, MKCSF, ML, MPLLG, TPO THRA thyroid hormone receptor, alpha ...

  10. mTOR Pathway

    Type
    Collection
    ...Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR associated protein; LST8 homolog LKB1 Also ... viral oncogene homolog FOXO Forkhead box O GBL/mLST8 MTOR associated protein; LST8 homolog GSK3 GSK3A...

  11. Ras Pathway

    Type
    Collection
    ...MET MET proto-oncogene, receptor tyrosine kinase MLST8 MTOR associated protein, LST8 homolog MTOR Mechanistic...

  12. Cre-lox system

    Type
    Collection
    ...CVS-N2c(deltaG)-Cre Cre CMV RABV Jessell 73717 pMLS328 Cre eft-3 C. elegans Jorgensen 75267 pAAV-CIBN-...
Showing: 1 - 12 of 12 results