We narrowed to 55 results for: MLS

...27 < 2–3 mL (~1 mL per site) Beginner Intradermal Anesthesia Between skin layers 26 < 0.05 mL Advanced... back or hind legs Gauge: 25–27 Volume: < 2–3 ml (~1 ml per site) Skill required: beginner Intradermal...–27 < 2–3 mL Intermediate Intramuscular Mechanical restrainer Thigh 25–27 < 0.02–0.05 mL Beginner ...cord 30 up to 0.01 mL Expert Intratracheal Anesthesia Trachea 20–23 (tubing) < 0.07 mL Advanced Intranasal...Nose n/a < 0.05 mL Beginner Oral gavage Scruffing Throat 18–20 (bulb tipped) up to 10 mL/kg Intermediate...vein or retro-orbitally Gauge: 27–30 Volume: < 0.2 ml Skill required: beginner (retro-orbital) to intermediate...Location: peritoneal cavity Gauge: 25–27 Volume: < 2–3 ml Skill required: intermediate Intramuscular Intramuscular...

...-100 ug/mL Ampicillin beta-lactam Inhibits cell wall synthesis Bactericidal 100-200 ug/mL Carbenicillin...Bactericidal 100 ug/mL Bleomycin glycopeptide Induces DNA breaks Bactericidal 5-100 ug/mL Erythromycin...subunit; causes mis-translation Bactericidal 50-100 ug/mL Spectinomycin aminoglycoside Binds 30S ribosomal...interrupts protein synthesis Bactericidal 7.5-50 ug/mL Streptomycin aminoglycoside Inhibits initiation...aminoacyl translocation Bacteriostatic 50-100 ug/mL in EtOH Polymyxin B polypeptide Alters outer ...outer membrane permeability Bactericidal 10-100 ug/mL Tetracycline tetracyclin Binds 30S ribosomal subunit...synthesis (elongation step) Bacteriostatic 10 ug/mL Chloramphenicol   Binds 50S ribosomal subunit; ...

...AAV Retrograde (AAVrg), and volumes of 1 mL, 2.5 mL, or 5 mL. Please note that all orders will be shipped...expect titers of 1 × 1013 GC/mL for AAV1, AAV5, AAV8, and AAVrg and 7 × 1012 GC/mL for AAV2. There are no quotes...

... Inhibits termination step of translation 2-10 ug/mL G418/Geneticin neo HeLa, NIH3T3, CHO, 293HEK, Jurkat...synthesis at 80S; inhibits chain elongation 100-800 ug/mL Hygromycin B hygB HeLa, NIH3T3, CHO, Jurkat T cells...synthesis at 80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK, Jurkat T cells Inhibits...protein synthesis; premature chain termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3, CHO, COS-1, 293HEK,...Complexes with DNA; causes strand scissions 100-400 ug/mL *Not comprehensive.   ** In eukaryotes.   ***The...

...transformant. I like to spin down 100 mL of culture and resuspend in 25 mL of transformation medium per Agrobacterium...density in 18-24 hours. I then add 0.5 mL of this starter culture to 100 mL fresh media, which is then ready...wide enough to dunk your plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are both great...stock or a colony from an LB plate. In my hands, a 5 mL starter culture inoculated from a colony on a plate...

...will turn yellow. Do NOT change the media (add 2-3 mL of fresh media once a week), and do NOT harvest the...days) Physical titer (particle count, rAdV genomes/mL) can be measured by: Optical density: particle concentration... of at 260nm corresponds to 1.1 x 1012 particles/mL. To calculate the particle number: OD260 reading ... x 1.1 x 1012 particles = number of particles per mL of sample. The purity of the sample can be estimated...Forming Units (PFU) or Infectious Units (IU), per mL)  Plaque formation assay: permissive cells are infected...

...different quantities: 10x 100 µL (1 mL), 25x 100 µL (2.5 mL), or 50x 100 µL (5 mL).  Once submitted, the request...

...drying out) while 3 ml of melted agarose are mixed with 3 ml of 2X DMEM in a 50 ml sterile tube (for 60... 60 mm dishes only 1.5 ml of each are used). This solution is capped, swirled and tested for the correct...

...to 50 ug/ml pure dsDNA. Use the following formula to estimate your DNA: Concentration (ug/ml) = A260 reading... reading x dilution factor x 50 ug/ml This method is quick and simple and doesn’t require any special ...

... a titer of 4 x 1012 genome copies/mL or 8 x 1012 genome copies/mL and both results would be valid. This...titers tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute your virus before adding...can then be used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies...

... 1012-1013 vg/ml (vector genomes per milliliter) and lentiviral vectors around 108 IU/ml (infectious units... units per ml)  in order to maximize possible effects. Injection Parameters: The injection needle should...

...yield** Miniprep 1–5 mL 5–50 µg Midiprep 25–100 mL 20–200 µg Maxiprep 100–500 mL 100–750 µg Megaprep...for example. These kits are designed to handle ~1–5 mL of liquid culture. Using a higher volume can affect...

...sources to increase protein yields from 36 μg/mL to 266 μg/mL. The reaction only required three components...

...concentration of antibiotics (ie. 50 μg/mL Amp instead of 100 μg/mL Amp), or using more nutrient rich media...

...20,000 lanes/50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing a...

...–701 . https://doi.org/10.1038/nbt0702-697 Hirsch ML, Agbandje-McKenna M, Jude Samulski R (2010) Little...:6–8 . https://doi.org/10.1038/mt.2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard...Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio A (2015) Improved dual ...

...8.5 x 10e10 GC/mL. The lot of viruses used (100798-AAV, lot #v15287, titer 1.7x10e13 GC/mL and 100799-AAV1...AAV1, lot # v14715, titer 2.4x10e13 GC/mL) for this study were packaged at Addgene and had a relatively...

...standard curve accordingly.    Figure 1: A 1 mg/mL control is diluted in a series ranging from 1:2 to...the trendline to extrapolate its x value, 0.963 mg/mL, from its y value, 0.651 nm.     How “good” ...

...polyethylene glycol (PEG). We add 25 mL of 40% PEG solution to each 100 mL of supernatant (final concentration...

... ensured to be less than five endotoxin units per mL. The endotoxin levels are determined using a chromogenic...-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression...