We narrowed to 2,306 results for: pET28

1. Kif1A-EGFP-SNAP

   Plasmid

   #229851

   Purpose

   Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

   Depositor

   William Hancock

   Insert

   Kif1A-EGFP-SNAP (Kif1a Rat)
   

   Use

   Tags

   6xHis, EGFP, and SNAP-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a(+)-T7-OmpA-MNaseA-6xHis

   Plasmid

   #214808

   Purpose

   Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

   Depositor

   Peter Sarin

   Article

   Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

   Insert

   OmpA-Micrococcal nuclease A
   

   Use

   Tags

   6xhis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-28a(+)-T7-OmpA-MNaseB-6xHis

   Plasmid

   #214809

   Purpose

   Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

   Depositor

   Peter Sarin

   Article

   Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

   Insert

   OmpA-Micrococcal nuclease B
   

   Use

   Tags

   6xhis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TCT-Universal plasmid

   Plasmid

   #221852

   Purpose

   A universal plasmid for expressing any protein with TetraCysteine motif

   Depositor

   Artur Krężel

   Article

   Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

   Insert

   Tetra Cysteine tag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SARS-CoV-2 Mpro protease (T190I mutant - clinical mutant); aliases: 3CLpro, 3C-like

   Plasmid

   #204786

   Purpose

   Bacterial expression of Sars Cov 2 MPRO T190I mutant

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T190I

   Promoter

   Available since

   Jan. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. SARS-CoV-2 Mpro protease (Q189K mutant - clinical mutant); aliases: 3CLpro, 3C-like

   Plasmid

   #204784

   Purpose

   Bacterial expression of Sars Cov 2 MPRO Q189K mutant

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q189K

   Promoter

   Available since

   Jan. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. SARS-CoV-2 Mpro protease (P132H mutant - clinical mutant); aliases: 3CLpro, 3C-like

   Plasmid

   #204783

   Purpose

   Bacterial expression of Sars Cov 2 MPRO P132H mutant

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   P132H

   Promoter

   Available since

   Jan. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. SARS-CoV-2 Mpro protease (N142S mutant - clinical mutant); aliases: 3CLpro, 3C-like

   Plasmid

   #204782

   Purpose

   Bacterial expression of Sars Cov 2 MPRO N142S mutant

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N142S

   Promoter

   Available since

   Jan. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. SARS-CoV-2 Mpro protease (M49T mutant - clinical mutant); aliases: 3CLpro, 3C-like

   Plasmid

   #204779

   Purpose

   Bacterial expression of Sars Cov 2 MPRO M49T mutant

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   M49T

   Promoter

   Available since

   Jan. 23, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. SARS-CoV-2 Mpro protease (M491I mutant - clinical mutant); aliases: 3CLpro, 3C-like

    Plasmid

    #204777

    Purpose

    Bacterial expression of Sars Cov 2 MPRO M49I mutant

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M49I

    Promoter

    Available since

    Jan. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. SARS-CoV-2 Mpro protease (G143S mutant - clinical mutant); aliases: 3CLpro, 3C-like

    Plasmid

    #204776

    Purpose

    Bacterial expression of Sars Cov 2 MPRO G143S mutant

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G143S

    Promoter

    Available since

    Jan. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. SARS-CoV-2 Mpro protease (A191V mutant - clinical mutant); aliases: 3CLpro, 3C-like

    Plasmid

    #204774

    Purpose

    Bacterial expression of Sars Cov 2 MPRO A191V mutant

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A191V

    Promoter

    Available since

    Jan. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. SARS-CoV-2 Mpro protease (A193T mutant - clinical mutant); aliases: 3CLpro, 3C-like

    Plasmid

    #204773

    Purpose

    Bacterial expression of Sars Cov 2 MPRO A193T mutant

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A193T

    Promoter

    Available since

    Jan. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. SARS-CoV-2 Mpro protease (A191T mutant - clinical mutant); aliases: 3CLpro, 3C-like

    Plasmid

    #204772

    Purpose

    Bacterial expression of Sars Cov 2 MPRO A191T mutant

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A191T

    Promoter

    Available since

    Jan. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. SARS-CoV-2 Mpro protease (A193S mutant - clinical mutant); aliases: 3CLpro, 3C-like

    Plasmid

    #204771

    Purpose

    Bacterial expression of Sars Cov 2 MPRO A193S mutant

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A193S

    Promoter

    Available since

    Jan. 23, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. SARS-CoV-2 Mpro protease (R188K mutant - clinical mutant); aliases: 3CLpro, 3C-like

    Plasmid

    #204770

    Purpose

    Bacterial expression of Sars Cov 2 MPRO R188K mutant

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R188K col1

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-like

    Plasmid

    #204769

    Purpose

    Bacterial expression of Sars Cov 2 MPRO C145S mutant

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C145S mutant

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. JPUB_006820

    Plasmid

    #87921

    Purpose

    E. coli expression of K. stuttgartiensis protein

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    Kuste2803
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JPUB_006826

    Plasmid

    #87923

    Purpose

    E. coli expression of K. stuttgartiensis protein

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    Kuste3608
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQuantEGFP-hygro-kana

    Plasmid

    #45588

    Depositor

    Andrzej Dziembowski

    Article

    Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

    Type

    Empty backbone

    Use

    Cre/Lox; Dt40 cell line targeting

    Tags

    Quant-EGFP tag

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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