Addgene

We narrowed to 2,789 results for: aen

Showing: 1401 - 1420 of 2789 results

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pMLS256

Plasmid

#73715

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

pU6::sgRNA

Use

CRISPR

Tags

Expression

Worm

Mutation

SapI insertion site

Promoter

C. elegans U6 snRNA pol III promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGC479

Plasmid

#42601

Depositor

Jane Hubbard

Article

Korta et al Development. 2012 Mar;139(5):859-70. doi

Insert

daf-15 (daf-15 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

T7

Available since

April 19, 2013

Availability

Academic Institutions and Nonprofits only
pCZGY2845

Plasmid

#87763

Purpose

Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pnmr-1-PH-miniSOG

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Pnmr-1

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2838

Plasmid

#87759

Purpose

Pmyo-3-PH::miniSOG, expression of membrane targeted PH-miniSOG in body wall muscles.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmyo-3-PH-miniSOG

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Pmyo-3

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pEG408

Plasmid

#40086

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (aa 345 - 468) (mex-5 Nematode)

Use

Tags

Dendra2 PFAF, S-peptide, and TEV protease cleavag…

Expression

Worm

Mutation

contains aa 345 - 468

Promoter

pie-1

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pRBBm69

Plasmid

#48116

Purpose

Shuttle vector E. coli/B.meg.

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

promoter sacB - sacB

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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AP575-1

Plasmid

#71277

Purpose

Expresses 3Xflag::tagRFP::myc in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagRFP

Use

Tags

3xFlag and myc

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pGC480

Plasmid

#42600

Depositor

Jane Hubbard

Article

Korta et al Development. 2012 Mar;139(5):859-70. doi

Insert

let-363 cDNA

Use

RNAi

Tags

Expression

Mutation

Promoter

T7

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pCZGY2842

Plasmid

#87762

Purpose

Punc-25-PH::miniSOG, expression of membrane targeted PH-miniSOG in GABAergic motor neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-25-PH-miniSOG

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Punc-25

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMB67

Plasmid

#47947

Purpose

Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Mutation

Promoter

hsp-16.48

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pMB63

Plasmid

#47945

Purpose

Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Mutation

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pRL542

Plasmid

#70282

Purpose

ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD4 GFP

Plasmid

#19181

Depositor

Al Fisher

Article

Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

Insert

Green fluorescent protein

Use

Tags

FLAG and daf-12 N-term 328 amino acids

Expression

Worm

Mutation

S65C, contains synthetic introns for C. elegans

Promoter

Available since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pAWp9

Plasmid

#73851

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-{BamHI+EcoRI}

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pRL2960

Plasmid

#70694

Purpose

pDU1-E. coli shuttle vector (Km-r) with extended polylinker

Depositor

Peter Wolk

Article

Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

Type

Empty backbone

Use

Bacterial shuttle vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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BSP608

Plasmid

#122253

Purpose

expression vector for mNeptune under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mNeptune

Use

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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XW82

Plasmid

#65840

Purpose

MosSCI-QUAS::Δpes10::GFP

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

5XQUAS-delta-pes-10::GFP

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pPM180

Plasmid

#64822

Purpose

GUS overexpression in planta

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

GUS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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TC550

Plasmid

#164891

Purpose

Punc-4::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

Depositor

Tian Chi

Article

Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

Insert

unc4-rtTA(Q)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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ploxP unc-119

Plasmid

#19185

Depositor

Al Fisher

Article

Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

Insert

unc-119 (unc-119 Nematode)

Use

Recombineering

Tags

Expression

Mutation

contains loxP site just 5' to ApaI site

Promoter

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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