We narrowed to 1,931 results for: cas9 expression vector

1. px458_Conc3

   Plasmid

   #134450

   Purpose

   Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ824

   Plasmid

   #39314

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. px459V2.0_Conc3

   Plasmid

   #134458

   Purpose

   Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX459-gR2A_Hinge

   Plasmid

   #124811

   Purpose

   Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Cas9 – gRNA_R2A_hinge
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Bacterial and Mammalian

   Mutation

   R166H in PuroR (please see depositors comment bel…

   Promoter

   pUC

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LT0

   Plasmid

   #210551

   Purpose

   Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   pU6::AAVS1gRNA::LT0::mCherrySSM
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. LT1

   Plasmid

   #210552

   Purpose

   Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   pU6::AAVS1gRNA::LT1::mCherrySSM
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LT2

   Plasmid

   #210553

   Purpose

   Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   pU6::AAVS1gRNA::LT2::mCherrySSM
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pShuttle.CC9

   Plasmid

   #192931

   Purpose

   Gateway compatible vector with BsaI cloning site and Cre-2A-Cas9 expression

   Depositor

   Thorsten Stiewe

   Article

   Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

   Type

   Empty backbone

   Use

   Gateway-compatible cloning vector

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. JK104: pMVP-DEST

   Plasmid

   #121851

   Purpose

   pMVP destination vector, empty expression plasmid backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Pmvp gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPPEDs

    Plasmid

    #162469

    Purpose

    protoplast test vector of pPPED

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Inserts

    Cas9H840A
    attR gateway destination cassette
    

    Use

    Prime editing destination vector for transient ex…

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Promoter

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPR-B_sgRNA-RMP24 (pAVA3547)

    Plasmid

    #239240

    Purpose

    Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting RMP24

    Depositor

    Andrei Alexandrov

    Article

    Che et al Cell Rep. 2025 Jun 24;44(6):115752. doi:

    Insert

    U6-driven sgRNA targeting RMP24
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX330-sgRNA006_CTCF

    Plasmid

    #232928

    Purpose

    Expression vector for a sgRNA against the mouse CTCF locus and SpCas9.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9 (Ctcf S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV/CMV-SCLM

    Plasmid

    #216758

    Purpose

    AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon

    Depositor

    George Augustine

    Insert

    SuperClomeleon followed by WPRE and human growth hormone polyA terminator
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    CMV (cytomegalovirus)

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGH224_sgRNA_2xMS2_Puro

    Plasmid

    #85413

    Purpose

    hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNA0304

    Plasmid

    #165612

    Purpose

    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    NotI site replaced the CAN1 spacer in parent vect…

    Promoter

    SNR52

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. IX001: pBS/Fiber

    Plasmid

    #131754

    Purpose

    Plasmid containing Ad5 Fiber gene flanked by homology arms for insertion into the recombinant Ad5 vector IQ013 to generate tropism modified vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Ad5 wild-type Fiber
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMJ806

    Plasmid

    #39312

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMJ826

    Plasmid

    #39316

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Histidine 840 to Alanine (H840A)

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMJ825

    Plasmid

    #39315

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A)

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMJ823

    Plasmid

    #39313

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9 (lin2744 Listeria innocua)
    

    Use

    CRISPR

    Tags

    MBP and thioredoxin-His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only