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Showing: 1401 - 1420 of 1749 results

  1. pcDNA3-endo-Nb80

    Plasmid
    #205518
    Purpose
    Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-Nb80
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205518-plasmid-map-sequence-id-409484

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  2. pcDNA3-endo-Nb37

    Plasmid
    #205514
    Purpose
    Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-Nb37
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205514-plasmid-map-sequence-id-409423

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  3. pcDNA3-nuc-Nb80

    Plasmid
    #205519
    Purpose
    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb80
    Use
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205519-plasmid-map-sequence-id-409422

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  4. pcDNA3-nuc-Nb37

    Plasmid
    #205515
    Purpose
    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb37
    Use
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205515-plasmid-map-sequence-id-409490

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  8. RFP-(SIM)3

    Plasmid
    #127148
    Purpose
    Mammalian expression plasmid for mCherry-(SIM)3 client to assess SUMO-SIM scaffolds.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    3 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127148-plasmid-map-sequence-id-255288

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  9. pAS132

    Plasmid
    #211503
    Purpose
    AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    mCherry
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211503-plasmid-map-sequence-id-417791

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  10. pH3mCHSH2

    Plasmid
    #101053
    Purpose
    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.
    Depositor
    Jennifer Lodge
    Article
    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108
    Insert
    Cryptococcus mCherry expression plasmid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Cryptococcus Histone3 promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    101053-plasmid-map-sequence-id-192942

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  11. pAAV-IV-3xFLAG/HA/NBL10

    Plasmid
    #110568
    Purpose
    Cre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tag
    Depositor
    Jeffrey Friedman, Alexander Nectow
    Insert
    3xFLAG/HA-tagged anti-GFP VHH
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    110568-plasmid-map-sequence-id-213334

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  13. pUT_mNF-Cas13d-Triplex

    Plasmid
    #227709
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d-Triplex
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-d2i
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    227709-plasmid-map-sequence-id-450930

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  15. attB53_SNCB+_attB53

    Plasmid
    #183614
    Purpose
    RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183614-plasmid-map-sequence-id-361603

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  18. pSR04

    Plasmid
    #69151
    Purpose
    read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV
    Depositor
    Sander van den Heuvel
    Article
    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10
    Inserts
    mCherry
    TagBFP
    eGFP
    Use
    Cre/Lox; Mossci
    Tags
    Expression
    Worm
    Mutation
    codon-optimzed index 1.0
    Promoter
    eef-1A.1 (eft-3) and rps-27
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69151-plasmid-map-sequence-id-125046

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  19. pUt-mNF-Cas13d

    Plasmid
    #224789
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-d2i
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224789-plasmid-map-sequence-id-445954

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  20. attB53_SNCB-_attB53

    Plasmid
    #183615
    Purpose
    RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183615-plasmid-map-sequence-id-366916

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Showing: 1401 - 1420 of 1749 results