We narrowed to 9,942 results for: plasmids 101
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USP08 (2GWF)
Plasmid#25217PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertusp08.hs.0001-0142 (USP8 Human)
UseTagsHisExpressionBacterialMutationrhodanese domain, residues 181-318PromoterAvailable sinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVH64-Tier1-PSV40-Citrine_p2a_SEAP
Plasmid#169558PurposeTier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).DepositorInsertPSV40-driven citrine-P2A-SEAP fusion construct
UseTagsExpressionMammalianMutationPromoterPSV40Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-BLU-VIPR
Plasmid#220497PurposeRetroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBLU-VIPR
Plasmid#220498PurposePlasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR; TransposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Chx10BP-LCL-EGFP
Plasmid#73992PurposeCre-dependent reporter plasmid for bipolar cells in the retinaDepositorInsertChx10BP-loxP-mCherry-loxP-EGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterChx10BP (chx10 enhancer)Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vGAT-VL-hIgG1-CL
Plasmid#218437Purposemammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domainDepositorInsertvGAT-VL-hIgG1-CL
UseAffinity Reagent/ AntibodyTagsBM40ExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 apmA
Plasmid#112881PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertapmA (apmA Staphylococcus aureus)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-Otx2-IRES-GFPd2
Plasmid#73996PurposePlasmid expressing Otx2(isoform b)-IRES-GFPd2DepositorInsertOtx2(isoform b)-Ires-GFP (Otx2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS2396-Tier1-PhCMV-4xCOX8_Ypet
Plasmid#169534PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).DepositorInsertPCMV-driven miochondria-localized yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-SDHAF3
Plasmid#136013PurposeSDHAF3 3'UTR (NM_020186.2) inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorInsertSDHAF3 3'UTR (ACN9 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS2410-Tier1-PhCMV-Myr_mTagBFP2
Plasmid#169535PurposeTier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).DepositorInsertPCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Oct4-Cr1
Plasmid#66989PurposeExpresses gRNA that targets stop codon for OCT4DepositorInsertgRNA for Crispr targeting the OCT4 stop codon
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 rmtB
Plasmid#112880PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertrmtB (RmtB Escherichia coli)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 ermC
Plasmid#112884PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInsertermC (ermM Staphylococcus epidermis)
UseTags6xHisExpressionBacterialMutationPromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 cat
Plasmid#112888PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertcat (Brevibacillus brevis) (cat Brevibacillus brevis)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-H2B-FusionRed
Plasmid#164430PurposeAAV plasmid expressing nuclear-localized FusionRed fluorophoreDepositorInsertH2B-FusionRed
UseAAVTagsH2BExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXU01
Plasmid#212151PurposePlasmid expressing xylitol dehydrogenase under inducible promoter PT7 control. Plasmid confers the ability to use xylitol as major carbon source to E. coli.DepositorInsertxylitol dehydrogenase
UseTags6x HisExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVH63-Tier1-PMLP-Citrine_p2a_SEAP
Plasmid#169557PurposeTier-1 vector encoding PMLP-driven Citrine-p2A-SEAP expression (PMLP-Citrine-p2A-SEAP-pA).DepositorInsertPMLP-driven citrine-P2A-SEAP fusion construct
UseTagsExpressionMammalianMutationPromoterPMLPAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only
