We narrowed to 1,440 results for: wac

1. DinoIII-pat

   Plasmid

   #155028

   Purpose

   Dinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, gene

   Depositor

   Senjie Lin

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Insert

   Phosphinothricin N-acetyltransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. DinoIII-bsr

   Plasmid

   #155027

   Purpose

   Dinoflagelate backbone for blasticidin S-resistance gene (bsr)

   Depositor

   Senjie Lin

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Insert

   Blasticidin-S deaminase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC:FCP:ShBle:FCP:EGFP

   Plasmid

   #85987

   Purpose

   Expresses ShBle and EGFP with FCP promoters/terminators for F. cylindrus transformation.

   Depositor

   Thomas Mock

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Inserts

   ShBle
   EGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   FCP

   Available since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA CRU HISMX

   Plasmid

   #131164

   Purpose

   PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Use

   Tags

   CRU

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJJB296

   Plasmid

   #107691

   Purpose

   35SPromoter-LwaCas13a-HSPTerminator

   Depositor

   Daniel Voytas

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   Cas13a
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJJB308

   Plasmid

   #107699

   Purpose

   AtU6 Promoter-LwaCas13a-gRNA Scaffold

   Depositor

   Daniel Voytas

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA CUP1p-NuiHA kanMX

   Plasmid

   #131169

   Purpose

   PCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiae

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Use

   Tags

   Nui-HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJJB302

   Plasmid

   #107694

   Purpose

   OsActin Promoter-LwaCas13a-HSP Terminator

   Depositor

   Daniel Voytas

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   Cas13a
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsActin

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA NuiHA-loxP-nat-CUP1p

   Plasmid

   #131170

   Purpose

   PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Use

   Tags

   Nui-HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDH5

    Plasmid

    #83775

    Purpose

    Useful for tagging yeast proteins with C-terminal YFP tag; his selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    YFP
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…

    Promoter

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDH3

    Plasmid

    #83789

    Purpose

    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    F64L, Y66W, N146I, M153T, V163A, N164H mutations …

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC019 - LwCas13a from Leptotrichia wadei

    Plasmid

    #91909

    Purpose

    Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a and guide expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pC014 - LwCas13a-msfGFP

    Plasmid

    #91902

    Purpose

    Expresses active LwCas13a for mammalian RNA targeting

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR

    Tags

    NLS and msfGFP-NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC035 - dLwCas13a-msfGFP

    Plasmid

    #91925

    Purpose

    Expresses catalytically inactive LwCas13a for mammalian RNA targeting

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    dLwCas13a
    

    Use

    CRISPR

    Tags

    NLS-3xHA and NLS-msfGFP

    Expression

    Mammalian

    Mutation

    R488A and R1060A

    Promoter

    EF-1a

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC029 - Lw2Cas13a from Leptotrichia wadei F0279

    Plasmid

    #91919

    Purpose

    Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    Lw2Cas13a and guide expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC034 - LwCas13a-msfGFP-2A-Blast

    Plasmid

    #91924

    Purpose

    Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC015 - dLwCas13a-NF

    Plasmid

    #91905

    Purpose

    Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    dLwCas13a
    

    Use

    CRISPR

    Tags

    NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

    Expression

    Mammalian

    Mutation

    R474A and R1046A

    Promoter

    CAG

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC011 LwCas13a locus with targeting spacer

    Plasmid

    #91903

    Purpose

    LwCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with targeting spacer
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. HEpl017

    Plasmid

    #179992

    Purpose

    pCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh promoter and tagmCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    TadA8e-dSiCas12i(D1049A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC012 LwCas13a locus with nontargeting spacer

    Plasmid

    #91904

    Purpose

    LwCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with non-targeting spacer
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge