CRISPR 101: Targeting RNA with Cas13a (C2c2)
Type
Blog Post
...distribution. When comparing LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency...characterized Cas13a from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize ...stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides...PFS, making it a very flexible cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5...response is well described in bacterial cells, LwaCas13a does not display nonspecific RNA cleavage activity...They subsequently used catalytically inactive dLwaCas13a as a programmable RNA-binding protein, creating...creating the fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging.
In short, the Cas13a molecule...