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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4 results for: wac

Showing: 1 - 4 of 4 results

  1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...distribution. When comparing LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency...characterized Cas13a from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize ...stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides...PFS, making it a very flexible cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5...response is well described in bacterial cells, LwaCas13a does not display nonspecific RNA cleavage activity...They subsequently used catalytically inactive dLwaCas13a as a programmable RNA-binding protein, creating...creating the fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging. In short, the Cas13a molecule...

  2. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(...Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998...

  3. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...flanking site (PFS) sequence, but orthologs like LwaCas13a do not require a specific PFS. Cas13 enzymes do...folded ortholog that cleaved RNA robustly, unlike LwaCas13a, which must be stabilized by monomeric superfolded...

  4. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...doi.org/10.1089/hyb.1988.7.453  Niemeyer CM, Adler M, Wacker R (2007) Detecting antigens by quantitative immuno-PCR...
Showing: 1 - 4 of 4 results