...distribution. When comparing LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency...characterized Cas13a from Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize ...stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides...PFS, making it a very flexible cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5...response is well described in bacterial cells, LwaCas13a does not display nonspecific RNA cleavage activity...They subsequently used catalytically inactive dLwaCas13a as a programmable RNA-binding protein, creating...creating the fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging. In short, the Cas13a molecule...

...Longtine MS, Shah NG, McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(...Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998...

...flanking site (PFS) sequence, but orthologs like LwaCas13a do not require a specific PFS. Cas13 enzymes do...folded ortholog that cleaved RNA robustly, unlike LwaCas13a, which must be stabilized by monomeric superfolded...

...doi.org/10.1089/hyb.1988.7.453  Niemeyer CM, Adler M, Wacker R (2007) Detecting antigens by quantitative immuno-PCR...