We narrowed to 2,958 results for: GRI;

1. pET28a-B4E

   Plasmid

   #162067

   Purpose

   For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E

   Depositor

   Karen Polizzi

   Article

   Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1

   Insert

   ampR-eIF4E fusion protein
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   eIF4E incorporating the K119A mutation, which is …

   Promoter

   T7

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJA167

   Plasmid

   #128536

   Purpose

   Expresses DsRed

   Depositor

   Marcos Alcocer

   Article

   Wang et al Mol Biotechnol. 2014 Feb;56(2):136-46. d

   Insert

   NFAT-DsRed reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40-NFAT

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_085

   Plasmid

   #158583

   Purpose

   Lentiviral expression of SpCas9; P2A site provides mKate2

   Depositor

   John Doench, David Root

   Article

   Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

   Insert

   SpCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-GLT1a

   Plasmid

   #162515

   Purpose

   Rat GLT-1a cDNA N-terminally tagged with EGFP

   Depositor

   Josef Kittler

   Article

   Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

   Insert

   GLT1a (Slc1a2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-CMV-TetO2_mVenus

   Plasmid

   #113893

   Purpose

   Mammalian (inducible) expression of cytosolic mVenus

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Insert

   monomeric Venus
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCVpuro BRIT1

   Plasmid

   #16205

   Depositor

   Shiaw-Yih Lin

   Article

   Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

   Insert

   BRIT1 (MCPH1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pBXNPH3

   Plasmid

   #110098

   Purpose

   E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDB_156

   Plasmid

   #216090

   Purpose

   Cas9 [Sp] CRISPRa targeting CD274, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD274 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. NLS-HA-tdMCP-tdTomato

   Plasmid

   #183938

   Purpose

   tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdMCP-tdTomato (cp Synthetic)
   

   Use

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 TEV-GFP-FLAG LIC cloning vector (6LD)

    Plasmid

    #166835

    Purpose

    Modified LIC 6D backbone with a C-terminal TEV-GFP-FLAG tag. Derived from Scott Gradia's pcDNA3.1 LIC 6D by Liron David.

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMV_AA090

    Plasmid

    #216099

    Purpose

    Knockout, EF1a-driven Cas12a (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas12a [EnAs]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-GLT1b

    Plasmid

    #162516

    Purpose

    Rat GLT-1b cDNA N-terminally tagged with EGFP

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1b (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AA345

    Plasmid

    #216031

    Purpose

    Fragmid fragment: (Cas protein) Cas13d

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas13d_v1.1 [Dj]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA009

    Plasmid

    #216037

    Purpose

    Fragmid fragment: (C' terminus) Nucleoplasmin NLS

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    NpNLS_v1.1; 3xHA_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_834

    Plasmid

    #216087

    Purpose

    Cas9 [Sp] knockout targeting CD59, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD59 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 CARD8

    Plasmid

    #169962

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 6A CARD8 CARD-FLAG WT

    Plasmid

    #164020

    Purpose

    Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO-puro-sh-mFAK C

    Plasmid

    #37015

    Depositor

    Bob Weinberg

    Article

    Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

    Insert

    sh-mFAK C (Ptk2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 31, 2012

    Availability

    Academic Institutions and Nonprofits only

19. rTetR-GFP-VP16

    Plasmid

    #183917

    Purpose

    VP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    rTetR-GFP-VP16 (UL48 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJM160

    Plasmid

    #134169

    Purpose

    Bacterial expression of RB3 stathmin-like domain (SLD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    RB3-SLD (STMN4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues 49-189

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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