We narrowed to 6,901 results for: plasmid dna

1. pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

   Plasmid

   #101207

   Purpose

   Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N497A/R661A/Q695A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N497A, R661A and Q695A

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

   Plasmid

   #101214

   Purpose

   Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/K855A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C and K855A

   Promoter

   T7

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Plasmid

   #101217

   Purpose

   Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

   Promoter

   T7

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

   Plasmid

   #101211

   Purpose

   Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

   Plasmid

   #101204

   Purpose

   Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

   Plasmid

   #101210

   Purpose

   Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

   Plasmid

   #101203

   Purpose

   Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…

   Promoter

   T7

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

   Plasmid

   #101212

   Purpose

   Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…

   Promoter

   T7

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A

   Plasmid

   #101181

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/H698A/Q926A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A

    Plasmid

    #101182

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/Q695A/H698A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

    Plasmid

    #101183

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    M694A/Q695A/H698A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

    Plasmid

    #101180

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
    

    Use

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid

    #101230

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Plasmid

    #101231

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…

    Promoter

    T7

    Available Since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Plasmid

    #101229

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Plasmid

    #101208

    Purpose

    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N497A, R661A and Q695A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Equalizer-L plasmid

    Plasmid

    #169732

    Purpose

    Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Equalizer-H plasmid

    Plasmid

    #169367

    Purpose

    Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Equalizer-M plasmid

    Plasmid

    #169731

    Purpose

    Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BGGLO QPCR PLASMID

    Plasmid

    #79905

    Purpose

    Encodes partial human gamma- and beta-globing cDNA for use as QPCR standard as published in PMID: 14592835..

    Depositor

    Jeffery Miller

    Article

    Bhanu et al Blood. 2004 Mar 1;103(5):1929-33. Epub 2

    Insert

    Human partial HBG and HBB gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    No promoter

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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