We narrowed to 6,459 results for: plasmid dna

1. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

   Plasmid

   #101210

   Purpose

   Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

   Plasmid

   #101203

   Purpose

   Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

   Plasmid

   #101212

   Purpose

   Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…

   Promoter

   T7

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A

   Plasmid

   #101181

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/H698A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A

   Plasmid

   #101182

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/Q695A/H698A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

   Plasmid

   #101183

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   M694A/Q695A/H698A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

   Plasmid

   #101180

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/Q695A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

   Plasmid

   #101230

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

   Plasmid

   #101231

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…

   Promoter

   T7

   Available since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Plasmid

    #101229

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Plasmid

    #101208

    Purpose

    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N497A, R661A and Q695A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Equalizer-L plasmid

    Plasmid

    #169732

    Purpose

    Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Equalizer-M plasmid

    Plasmid

    #169731

    Purpose

    Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Equalizer-H plasmid

    Plasmid

    #169367

    Purpose

    Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Integrated Genomics Plasmids

    Plasmid Kit

    #1000000004

    Purpose

    Plasmids used in Integrated Genomics: A Discovery-Based Laboratory Course; please note that the C. elegans yeast two-hybrid cDNA library is unavailable

    Depositor

    Guy Caldwell

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

16. BGGLO QPCR PLASMID

    Plasmid

    #79905

    Purpose

    Encodes partial human gamma- and beta-globing cDNA for use as QPCR standard as published in PMID: 14592835..

    Depositor

    Jeffery Miller

    Article

    Bhanu et al Blood. 2004 Mar 1;103(5):1929-33. Epub 2

    Insert

    Human partial HBG and HBB gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    No promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Negative feedback (NF) plasmid

    Plasmid

    #169747

    Purpose

    Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tetO2

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Incoherent feedforward (IFF) plasmid

    Plasmid

    #169748

    Purpose

    Standalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    miR(FF4) target-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tetO2

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPSU2

    Plasmid

    #89566

    Purpose

    High copy number plasmid 2 to prepare Penn State DNA molecular weight ladders

    Depositor

    Song Tan

    Article

    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.

    Inserts

    750 bp EcoRV fragment
    3000 bp EcoRV fragment
    4000 bp EcoRV fragment
    50 bp PstI fragment
    100 bp PstI fragment
    200 bp PstI fragment
    300 bp PstI fragment
    400 bp PstI fragment
    500 bp PstI fragment
    600 bp PstI fragment
    1500 bp Psti fragment
    4100 bp PstI fragment
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV+ plasmid with onboard mCherry cassette

    Plasmid

    #169741

    Purpose

    CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge