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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,524 results for: poly

Showing: 1421 - 1440 of 6524 results

  1. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P80C

    Plasmid
    #114602
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P80C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Promoter
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114602-plasmid-map-sequence-id-224743

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  2. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-A60C

    Plasmid
    #114601
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, A…
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114601-plasmid-map-sequence-id-222795

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  3. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P70C

    Plasmid
    #114605
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P70C cysteine mutations and a 42 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114605-plasmid-map-sequence-id-222758

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  4. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-A60C

    Plasmid
    #114604
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 42 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, A…
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114604-plasmid-map-sequence-id-222736

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  5. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P90C

    Plasmid
    #114603
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114603-plasmid-map-sequence-id-222733

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  7. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

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  8. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

    Plasmid
    #99694
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    99694-plasmid-map-sequence-id-194587

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  9. FUW-C-prM-E-NS1

    Plasmid
    #175281
    Purpose
    lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP.
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    C-prM-E-NS1 and EGFP (POLY Yellow fever virus strain 17D)
    Use
    Lentiviral
    Tags
    IRES EGFP
    Expression
    Mutation
    Promoter
    human ubiquitin C
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175281-plasmid-map-sequence-id-349091

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  10. AAV-Syn-NS1

    Plasmid
    #175276
    Purpose
    AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    NLS-dTomato (P2A cleavage)
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175276-plasmid-map-sequence-id-349086

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  12. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid
    #99690
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Sept. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    99690-plasmid-map-sequence-id-194510

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  13. pAAV-DIO-NS1

    Plasmid
    #175273
    Purpose
    AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175273-plasmid-map-sequence-id-346765

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  14. AAV-TRE-NS1-dTomato

    Plasmid
    #175278
    Purpose
    AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    NS1; dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    dTomato (from bidirectional promoter)
    Expression
    Mutation
    Promoter
    bidirectional TRE promoter
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175278-plasmid-map-sequence-id-349702

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  18. TFORF1953

    Plasmid
    #142897
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PARP1 (PARP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    142897-plasmid-map-sequence-id-427319

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  19. pHIPH4

    Plasmid
    #117685
    Purpose
    Hansenula polymorpha expression plasmid
    Depositor
    Ida van der Klei
    Insert
    Alcohol Oxidase promoter
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Alcohol Oxidase
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    117685-plasmid-map-sequence-id-232223

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  20. pETM41-EcPPK

    Plasmid
    #38334
    Depositor
    Florian Freimoser
    Insert
    E. coli polyphosphate kinase (ppk Escherichia coli)
    Use
    Tags
    6xHIS, MBP, and TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 1421 - 1440 of 6524 results