We narrowed to 1,545 results for: Tef;

1. 5HT2C-RNA1-FLAG

   Plasmid

   #79678

   Purpose

   serotonin receptor 2C RNA isoform 1 with C-terminal flag tag

   Depositor

   Stefan Stamm

   Article

   Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

   Insert

   5HT2C-RNA1 (HTR2C Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pETM33_USP7_MATH

   Plasmid

   #178490

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   USP7_MATH (USP7 Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQHA-USP7 CS puroR

   Plasmid

   #46754

   Purpose

   Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

   Depositor

   Gordon Peters, Goedele Maertens

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C223S--catalytically inactive

   Promoter

   CMV

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. tau exon 9–12 splicing minigene

   Plasmid

   #121165

   Purpose

   Generates circular RNAs from a Tau minigene

   Depositor

   Stefan Stamm

   Article

   Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

   Insert

   Tau exons 9–12 (MAPT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSN068

   Plasmid

   #101749

   Purpose

   Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIV537

   Plasmid

   #177704

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   tRNA-sgRNA-tRNA
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-NUP188

   Plasmid

   #159893

   Purpose

   Expresses NUP188 in mammalian cells

   Depositor

   Kozo Tanaka

   Article

   Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

   Insert

   NUP188 (NUP188 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   To avoid NotI site, first three amino acids are d…

   Promoter

   CMV

   Available since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. WDR5-HaloTag

   Plasmid

   #200881

   Purpose

   Mammalian expression of human WDR5 with C-terminal HaloTag fusion

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

   Insert

   WDR5-HaloTag (WDR5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-FLAG-hEcto/Tif1g-CAmut

   Plasmid

   #20903

   Depositor

   Stefano Piccolo

   Article

   Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

   Insert

   Ectodermin (TRIM33 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Point mutagenesis of C125 and C128 to Alanine (RI…

   Promoter

   Available since

   May 15, 2009

   Availability

   Academic Institutions and Nonprofits only

10. SPOP-V5 (BTB+BACK)

    Plasmid

    #169723

    Purpose

    Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    L186D, L190D, L193D, I217K, Y353E

    Promoter

    CMV

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUDE731

    Plasmid

    #103008

    Purpose

    S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    Fncpf1
    

    Use

    Tags

    3HA and NLS

    Expression

    Yeast

    Mutation

    human codon optimized

    Promoter

    TEF1

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_SNORD27h_115mut

    Plasmid

    #73068

    Purpose

    expression clone for human mutated SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (SNORD27 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    expression cassette for SNORD27 with AS box from …

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pToxamEXP4CUP1

    Plasmid

    #218284

    Purpose

    Use together with pJE13HD7 and pJE13HD7 derivatives to integrate RelE expression cassette under the control of the CUP1 promoter at ho locus

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    Hph>tAgTEF1-ISceI-Repeat2-pCUP1>RelE(1-125)>RPL28intron>RelE(126, 289)>tTPI1-HO(1828, 1979)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA 3.1 - circMAN2A1

    Plasmid

    #198837

    Purpose

    This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

    Depositor

    Stefan Stamm

    Article

    Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

    Insert

    circular mannosidase alpha class 2A member 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    circular RNA composed by 2 exons

    Promoter

    CMV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNIC28-HiBIT-WDR5

    Plasmid

    #200883

    Purpose

    Bacterial expression of N-terminal HiBIT-tagged human WDR5 protein

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    HiBIT-WDR5 (WDR5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGTVL2-BIRC2-BIR3

    Plasmid

    #196367

    Purpose

    protein expression of the GST-tagged BIR3 domain

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Front Cell Dev Biol. 2022 May 26;10:8865

    Insert

    BIRC2 BIR3 domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only S260-Y352

    Promoter

    T7

    Available since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAFMW-Nbr[R272A,K276A, R280A,K284A]

    Plasmid

    #188599

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R272A [CGG=> GCG], K276A [AAG=>GCG], R280A …

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBY011-AMN1ko

    Plasmid

    #183099

    Purpose

    S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:

    Insert

    AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
    

    Use

    Tags

    KanMX

    Expression

    Bacterial and Yeast

    Mutation

    only includes external sequences (504 bp each) as…

    Promoter

    URA3, TEF1

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. EC2_2_dCas9_VP64_sgRNA

    Plasmid

    #163707

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VP64+SV40 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-N-Myc_WT USP7

    Plasmid

    #131242

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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