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R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166014

Purpose

mammalian expression of HCoV-HKU1 soluble spike trimer protein

Depositor

Dominic Esposito

Publication

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-HKU1 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pInducer20 EGFP-TEADi

Plasmid

#140145

Purpose

Tetracycline-inducible TEADi (GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal)

Depositor

Ramiro Iglesias-Bartolome

Publication

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

EGFP TEADi

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pinducer 20 DN-KASHΔPPPL

Plasmid

#129280

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL

Depositor

Daniel Conway

Publication

Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(1

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Mutation

the last 4 amino acids (PPPL) of the KASH domain …

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLX311-Cas9

Plasmid

#118018

Purpose

Constitutive expression of Cas9

Depositor

William Hahn, David Root

Publication

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Cas9

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pYPQ141-ZmUbi-RZ-As

Plasmid

#86196

Purpose

AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

Depositor

Yiping Qi

Publication

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

AsCpf1 gRNA cloning site for ribozyme cleavage

Use

CRISPR

Tags

Hammerhead ribozyme and HDV ribozyme

Expression

Plant

Promoter

Maize ubiquitin 1

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGAMA-empty

Plasmid

#74755

Purpose

Mammalian lentiviral vector

Depositor

Miguel Ramalho-Santos

Publication

Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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All_in_one_CRISPR/Cas9_LacZ

Plasmid

#74293

Purpose

"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening

Depositor

Lynne Postovit

Publication

Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do

Inserts

LacZ-alpha
Cas9
mCherry

Use

CRISPR

Tags

HA

Expression

Bacterial and Mammalian

Promoter

Lac promoter, SV40, and T7 promoter

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-BRPF1

Plasmid

#65382

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRPF1 (BRPF1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
GFP-BRD1

Plasmid

#65375

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD1 (BRD1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pThy1-Autobow

Plasmid

#45187

Depositor

Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pThy1-Autobow

Use

Linearized for transgenic mouse injection

Promoter

Thy1

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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Active Wnt4-V5

Plasmid

#43812

Depositor

Xi He

Publication

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT4 (WNT4 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHMGWA-Pa14Csy4

Plasmid

#41091

Depositor

Jennifer Doudna

Publication

Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

Insert

Csy4

Tags

His6-MBP

Expression

Bacterial

Mutation

Wild type

Promoter

T7

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pENTR221 LRRK2 wt

Plasmid

#39529

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Gateway compatible cloning vector

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pDONR223-ADRBK1

Plasmid

#23835

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ADRBK1 (GRK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-RIPK2

Plasmid

#23846

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIPK2 (RIPK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-HCK

Plasmid

#23879

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HCK (HCK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-LCK

Plasmid

#23890

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LCK (LCK Human)

Use

Gateway Cloning

Mutation

V93I

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-BMPR1A

Plasmid

#23618

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BMPR1A (BMPR1A Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
GFP-GW182delta1

Plasmid

#11592

Depositor

Edward Chan

Publication

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

GW182 (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contains only aa1-498; SEE DEPOSITOR NOTES BELOW

Available since

June 30, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONRp5-p2 GFP-NShroom3-iLID

Plasmid

#170976

Purpose

N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.

Depositor

Miki Ebisuya

Publication

Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

Insert

NShroom3 (Shroom3 Mouse)

Use

Gateway Cloning

Tags

eGFP and iLID

Mutation

From isoform 2, deleted amino acids 1389 - 1805,…

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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