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Showing: 1461 - 1480 of 2071 results

  7. pCMV-eGFP-mPlum-HA

    Plasmid
    #200009
    Purpose
    Donor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.
    Depositor
    Thomas Noll
    Article
    Hertel et al Front Bioeng Biotechnol. 2022 Oct 13;10:
    Insert
    eGFP, mPlum
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200009-plasmid-map-sequence-id-398431

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  19. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Expression
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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Showing: 1461 - 1480 of 2071 results