We narrowed to 10,414 results for: 158

1. pCAH01

   Plasmid

   #128162

   Purpose

   IncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteria

   Depositor

   Michael Guarnieri, Calvin Henard

   Article

   Henard et al Sci Rep. 2016 Feb 23;6:21585. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPuro3.1(+)_Strep-Lrrk2-Myc

   Plasmid

   #161583

   Purpose

   expression of full-length mouse Lrrk2 with C-terminal Myc tag

   Depositor

   Maik Hintze

   Insert

   Lrrk2 (Lrrk2 Mouse)
   

   Use

   Tags

   Myc tag and StrepII

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC-HIS6-GST-TEV-AVITAG-GG

   Plasmid

   #215809

   Purpose

   Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pSuper-Rem2-1

   Plasmid

   #51584

   Purpose

   shRNA 1 against rodent Rem2

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

   Insert

   Rem2 (Rem2, Rem2 Rat, Mouse)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. ACSM2B

   Plasmid

   #39167

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ACSM2B (ACSM2B Human)
   

   Use

   Baculovirus expression

   Tags

   His Tev

   Expression

   Mutation

   Actually the mitochondrial form, GI:58082049, wit…

   Promoter

   polyhedrin

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. PECR

   Plasmid

   #39158

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PECR (PECR Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pNIC-HIS6-SUMO1-AVITAG-GG

   Plasmid

   #215808

   Purpose

   Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitag

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pJK502

   Plasmid

   #71582

   Purpose

   Produces Acetobacter aceti 1023 putative SixA with C-terminal His6 tag (SixAH6)

   Depositor

   T. J. Kappock

   Article

   Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

   Insert

   phosphohistidine phosphatase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSGE_773_zen_VRE_dCP

   Plasmid

   #71158

   Purpose

   Luciferase reporter

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   773_zen_VRE_dCP
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Insect

   Mutation

   Promoter

   DSCP Drosophila synthetic core promoter

   Available since

   Nov. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiCRISPRv2-Opti

    Plasmid

    #163126

    Purpose

    LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHoss1

    Plasmid

    #63158

    Purpose

    Suicide plasmid for efficient gene mutation in Gram-positive bacteria

    Depositor

    Attila Karsi

    Article

    Abdelhamed et al Plasmid. 2015 May 31;81:1-8. doi: 10.101

    Insert

    anti-secY-Pxyl/tetO-tetR
    

    Use

    Suicide plasmid for gram-positive bacteria

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid

    #118158

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc

    Plasmid

    #161585

    Purpose

    expression of Myc-tagged mouse Lrrk2 containing Parkinson's associated G2019S mutation

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    G2019S

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-tdMCP-12xGCN4

    Plasmid

    #205158

    Purpose

    express tdMCP-12xGCN4

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    tdMCP-12xGCN4
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc

    Plasmid

    #161584

    Purpose

    expression of Myc-tagged kinase-dead mouse Lrrk2 containing D1994S mutation

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    D1994S

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)_Strep-Lrrk2-Myc

    Plasmid

    #161582

    Purpose

    expression of full-length mouse Lrrk2 with C-terminal Myc tag

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMV306hsp+FFlucWT

    Plasmid

    #26158

    Depositor

    Brian Robertson, Siouxsie Wiles

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks the last 3 aa.

    Promoter

    Available since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAMBIA-FLS2prom::FLS2-3xmyc

    Plasmid

    #86158

    Purpose

    FLS2 expression

    Depositor

    Silke Robatzek

    Article

    Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Use

    Tags

    3xmyc

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS316 GAL cluster

    Plasmid

    #61925

    Purpose

    Expression of GAL1 and GAL10 from S. cerevisiae cloned into pRS316

    Depositor

    Jon Houseley

    Article

    Cruz et al Elife. 2014 Feb 11;3:e01581. doi: 10.755

    Inserts

    GAL1 (GAL1 Budding Yeast)
    GAL10 (GAL10 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc

    Plasmid

    #161587

    Purpose

    expression of Myc-tagged mouse Lrrk2 without GTPase domain

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    deletion: AA1023-1879

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge