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pTK586

Plasmid

#114716

Purpose

SNAP-tagging at LGN's N-terminal locus (BSD)

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTK473

Plasmid

#114715

Purpose

sgRNA for LGN's N-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

LGN sgRNA

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTK421

Plasmid

#114688

Purpose

RFP-Nano tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

RFP-Nano

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAN-OR

Plasmid

#62307

Purpose

synthetic circuit: OR gate

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
A4NT sgRNA
A2NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PA2, PPhlF, and pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pG-01

Plasmid

#166932

Purpose

Toehold switch part (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Toehold Switch - Block G - Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pE-01

Plasmid

#166930

Purpose

Stop codon part (E)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

STOP CODON TAA and spacer - BLOCK E- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pC-01

Plasmid

#166925

Purpose

sRNA part (C)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

sRNA ToeHold - Block C- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pB-01

Plasmid

#166924

Purpose

Ptac promoter part (B)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

PTAC promoter - Block B- Flanked by BsaI restriction sites.

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pA-01

Plasmid

#166923

Purpose

LacI expression cassette (A)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

LacI expression cassette - Block A- Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_440

Plasmid

#100497

Purpose

Mammalian expression of E. coli LF82_440 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_440

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_670

Plasmid

#100460

Purpose

Mammalian expression of E. coli LF82_670 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_670

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pWT050d

Plasmid

#107901

Purpose

W2.5-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T) and is part of CAMERA 2.5

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T)

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT046e

Plasmid

#107900

Purpose

W2.5-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C) and is part of CAMERA 2.5

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C)

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT062a

Plasmid

#107909

Purpose

W2m.2-2 plasmid. Contains UBC-TetR-P2A-LacI and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

UBC-TetR-P2A-LacI

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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EKv408(Gold Cas-guide insert4)

Plasmid

#248337

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv407(Gold Cas-guide insert3)

Plasmid

#248336

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv406(Gold Cas-guide insert2)

Plasmid

#248335

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv410(Gold Cas-guide insert6)

Plasmid

#248339

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv411(Gold Cas-guide insert7)

Plasmid

#248340

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv412(Gold Cas-guide insert8)

Plasmid

#248341

Purpose

gRNA insert vector for Golden Gate Assembly

Depositor

Elena Kozgunova

Article

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge