We narrowed to 6,838 results for: pcr

1. pFA6a-kanMX6

   Plasmid

   #39296

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. AbVec2.0-IGHG1

   Plasmid

   #80795

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AbVec1.1-IGKC

   Plasmid

   #80796

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-His3MX6

   Plasmid

   #41596

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-3HA-His3MX6

   Plasmid

   #41600

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, A. gossypii translation elongation factor 1…

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-GFP(S65T)-TRP1

   Plasmid

   #41597

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GFP (S65T) and yeast ADH1 terminator

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5 HA3-eDHFR-T2A-puro

   Plasmid

   #86395

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A puro
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A puro

   Expression

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMH5

   Plasmid

   #52531

   Purpose

   expresses FLP recombinase in Drosophila

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   FLP recombinase (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   ubiquitin

   Available since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAc5 HA3-eDHFR-T2A-neo

   Plasmid

   #86396

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A neo
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A neo

   Expression

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-TRP1-PGAL1-3HA

    Plasmid

    #41609

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, TRP1 reverse orientation, and yeast ADH1 te…

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-TRP1-PGAL1-GFP

    Plasmid

    #41615

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP(S65T), TRP1 reverse orientation, and yeast AD…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAH1

    Plasmid

    #41825

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    T7, TEF

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-TRP1-PGAL1-GST

    Plasmid

    #41612

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GST, TRP1 reverse orientation, and yeast ADH1 ter…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKF251

    Plasmid

    #52532

    Purpose

    Double-GFP reporter for DNA cleavage

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Double-GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available since

    April 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-SRM (sortase recognition motif)-kanMX6

    Plasmid

    #54489

    Purpose

    PCR based C-terminal SRM tagging in yeast

    Depositor

    Eri Sakata

    Insert

    SRM (sortase-recognition motif)
    

    Use

    Yeast genome targeting

    Tags

    kanMX6

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

16. ACT1-CUP1 9bp dist

    Plasmid

    #234131

    Purpose

    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

    Depositor

    Aaron Hoskins

    Insert

    ACT1-CUP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Intron truncation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ACT1-CUP1 12bp dist

    Plasmid

    #234130

    Purpose

    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

    Depositor

    Aaron Hoskins

    Insert

    ACT1-CUP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Intron truncation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ACT1-CUP1 27bp dist

    Plasmid

    #234129

    Purpose

    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

    Depositor

    Aaron Hoskins

    Insert

    ACT1-CUP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Intron truncation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ACT1-CUP1 15bp dist

    Plasmid

    #234128

    Purpose

    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

    Depositor

    Aaron Hoskins

    Insert

    ACT1-CUP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Intron truncation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ACT1-CUP1 42bp dist

    Plasmid

    #234127

    Purpose

    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

    Depositor

    Aaron Hoskins

    Insert

    ACT1-CUP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Intron truncation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge