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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,559 results for: pcr

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Showing: 1461 - 1480 of 7559 results

  8. pEasyG3_mic

    Plasmid
    #184920
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184920-plasmid-map-sequence-id-368413

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  9. pEasyG2_mic

    Plasmid
    #184916
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184916-plasmid-map-sequence-id-371408

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  10. pOpen-ETSSB

    Plasmid
    #165549
    Purpose
    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Extreme Thermostable Single-Stranded DNA Binding Protein
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165549-plasmid-map-sequence-id-339430

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  11. pOpen-BovDNTT

    Plasmid
    #165580
    Purpose
    Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bovine DNTT
    Use
    Synthetic Biology
    Available Since
    July 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165580-plasmid-map-sequence-id-338558

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Showing: 1461 - 1480 of 7559 results