Addgene

We narrowed to 2,080 results for: 3-Oct

Showing: 1521 - 1540 of 2080 results

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pAPM-D4 miR30-SUV39h1 ts2

Plasmid

#115881

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h1 ts1

Plasmid

#115880

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CREB

Plasmid

#68550

Purpose

AAV expression of CREB

Depositor

Eric Nestler

Article

Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

Insert

CREB

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pscALPS-HIV-1 Group P-vpr

Plasmid

#115837

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group P Vpr (HQ179987)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L

Plasmid

#89576

Purpose

caCas9 integrating vector into the Neut5L locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pscALPS-HIV-1 Group O-vpr

Plasmid

#115836

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group O Vpr (L20571)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-mCREB

Plasmid

#68551

Purpose

AAV expression of mCREB

Depositor

Eric Nestler

Article

Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

Insert

mCREB

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

S133A

Promoter

CMV

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only
pZ005 (AacC2c1 human codon optimized protein)

Plasmid

#70171

Purpose

Expresses AacC2c1 human codon optimized protein

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

AacC2c1 protein

Use

Tags

NLS

Expression

Mutation

Promoter

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pZ004 (LseC2c2 locus)

Plasmid

#70170

Purpose

Expresses LseC2c2 locus with one spacer in array

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

LseC2c2 locus with one spacer in array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
pClneoFH-let60 G12V

Plasmid

#66860

Purpose

Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

Let-60 (let-60 Nematode)

Use

Tags

FLAG-His6

Expression

Mammalian

Mutation

Glycine 12 is mutated to valine

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pGex4t2 with full-length SUUR

Plasmid

#112990

Purpose

Expresses fusion of GST and full-length Drosophila SUUR

Depositor

Terry Orr-Weaver

Article

Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

Insert

SuUR (SuUR Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET45 His-proSAAS (21 kDa)

Plasmid

#231636

Purpose

Bacterial expression of 21 kDa mouse proSAAS chaperone protein

Depositor

Iris Lindberg

Article

Hoshino et al J Neurochem. 2014 Feb;128(3):419-30. doi

Insert

Pcsk1N (Pcsk1n Mouse)

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h1 ts3

Plasmid

#115882

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pClneoEGFPC-let60 K16N

Plasmid

#66861

Purpose

Express GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

Let-60 (let-60 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lysine 16 is mutated to asparagine

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pmCherry-Gal3

Plasmid

#85662

Purpose

Fluorescent reporter for lysosomal damage

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

LGALS3 (LGALS3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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PM-RA

Plasmid

#211705

Purpose

Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cells

Depositor

Yasunori Saheki

Article

Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

Insert

PM-targetted RA (Lck Synthetic)

Use

Tags

RA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCWXPG-UBI-Ngn3PDX1MafA

Plasmid

#114303

Purpose

Constitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptides

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Insert

Ngn3 (NEUROG3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Ngn3, PDX1 and MafA proteins are expressed from a…

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-hDKK3-flag

Plasmid

#15496

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK3 (DKK3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

See note below

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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pDY1462_SERPINA1_CCA32_Caspase

Plasmid

#193194

Purpose

Express Caspase conditional upon RNA transcript

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

iCaspase9 (CASP9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-EF1.Pro

Plasmid

#79488

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

EF1 Promoter (EEF1A1 Synthetic, Human)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

EF1 Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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