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pMK-PatpB (NEP)

Plasmid

#123467

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

PatpB (NEP)

Use

Synthetic Biology

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMK-PaccD (functional)

Plasmid

#123465

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

PaccD (functional)

Use

Synthetic Biology

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLIC.B3.CT0478

Plasmid

#11526

Depositor

Sung-Hou Kim

Article

Kim et al Proteins. 2006 Feb 1. 62(2):322-8.

Insert

ScpB

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pMpGWB403-Lifeact-Citrine

Plasmid

#100582

Purpose

Binary vector for expression of Citrine-fused Lifeact (Lifeact-Citrine) to visualize actin filaments in plants

Depositor

Yutaka Kodama

Article

Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

Insert

Lifeact-Citrine

Expression

Plant

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRL443

Plasmid

#70261

Purpose

Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Type

Empty backbone

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-tTA

Plasmid

#104102

Purpose

Expresses tTA2 under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tTA2

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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TOP-GFP.mC

Plasmid

#35491

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xTOP-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

7xTCF/LEF

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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TOP-GFP

Plasmid

#35489

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xTOP-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

7xTCF/LEF

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pLPT20

Plasmid

#85460

Purpose

Repressilator with integrated reporter

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Inserts

mCfp
mVenus
tetR
lacI
cI

Use

Synthetic Biology

Tags

ssrA

Expression

Bacterial

Promoter

PLlac, PLtet, PR, and PRNAI

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pColE1_70a_deGFP_AmpR

Plasmid

#119161

Purpose

Expresses deGFP from a strong sigma 70 promoter

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

deGFP

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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DVC_pBR322_AF

Plasmid

#217598

Purpose

Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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DVC_p15A_AF

Plasmid

#217588

Purpose

Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pECGMC3

Plasmid

#75441

Purpose

Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mf-lon

Expression

Bacterial

Promoter

nisA promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Ddo_piwi-1

Plasmid

#107628

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

piwi-1

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Ddo_sfrp-1

Plasmid

#107637

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

sfrp-1

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Ddo_beta-catenin-1

Plasmid

#107639

Purpose

RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

beta-catenin-1

Use

RNAi

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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Ddo_porcupine-1

Plasmid

#107631

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

porcupine-1

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Ddo_innexin-10

Plasmid

#107633

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

innexin-10

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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piRFP702-N1

Plasmid

#45456

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP702

Expression

Mammalian

Mutation

T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pJethlpAmNeonGreenKm

Plasmid

#206816

Purpose

Template for hlpA-mneongreen amplification associated with kanamycin resistance gene

Depositor

Virginie Libante

Article

Mignolet et al bioRxiv 2025.05.10.653270

Insert

hlpA

Use

Synthetic Biology

Tags

mNeonGreen

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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