We narrowed to 15,528 results for: alp;
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Plasmid#50486Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsGFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the GFP open…PromoterCMVAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-GFP
Plasmid#50486Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsGFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the GFP open…PromoterCMVAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSCALPs-HA-NFAT1 (4-460)-GFP
Plasmid#88879PurposeLentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFPDepositorInsertHA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
UseLentiviralTagsGFP fusion protein and HA tagExpressionMammalianMutationamino acids 4-460 only; L78P & L287P *see bel…PromoterSFFV (spleen focus forming virus)Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSCALPs-HA-NFAT1 (4-460)-GFP
Plasmid#88879PurposeLentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFPDepositorInsertHA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
UseLentiviralTagsGFP fusion protein and HA tagExpressionMammalianMutationamino acids 4-460 only; L78P & L287P *see bel…PromoterSFFV (spleen focus forming virus)Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA6-11 1397C
Plasmid#221938PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6/11 1397CDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGMutationTALE: Q231R, W232G, S233A | DddA: T1413IAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA6 1397N
Plasmid#221939PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGMutationTALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…Available SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA11 1397N
Plasmid#221940PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA11 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGMutationTALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…Available SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA6-11 1397C
Plasmid#221938PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6/11 1397CDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGMutationTALE: Q231R, W232G, S233A | DddA: T1413IAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA6 1397N
Plasmid#221939PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGMutationTALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…Available SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA11 1397N
Plasmid#221940PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA11 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGMutationTALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…Available SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1alpha-soCoChR-tdTomato
Plasmid#107714PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoterDepositorInsertsoCoChR-tdTomato
UseAAVTagsKA2 and tdTomatoExpressionMammalianPromoterEF1alphaAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1alpha-soCoChR-tdTomato
Plasmid#107714PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoterDepositorInsertsoCoChR-tdTomato
UseAAVTagsKA2 and tdTomatoExpressionMammalianPromoterEF1alphaAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha1 clone1
Plasmid#162119PurposeLentiviral sgRNA plasmid targeting human AMPK alpha1DepositorInsertsgAMPK alpha1 (PRKAA1 Human)
UseLentiviralAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgAMPK alpha1 clone1
Plasmid#162119PurposeLentiviral sgRNA plasmid targeting human AMPK alpha1DepositorInsertsgAMPK alpha1 (PRKAA1 Human)
UseLentiviralAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-mCherry
Plasmid#50480Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsmCherry fusion in M3-M4 loop (after residue A405)ExpressionMammalianPromoterCMVAvailable SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-mCherry
Plasmid#50480Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
TagsmCherry fusion in M3-M4 loop (after residue A405)ExpressionMammalianPromoterCMVAvailable SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pscALPS gag-gfp/vpx
Plasmid#115808PurposeSFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpxDepositorInsertgag-gfp
UseLentiviralPromoterSFFVAvailable SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS gag-gfp/vpx
Plasmid#115808PurposeSFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpxDepositorInsertgag-gfp
UseLentiviralPromoterSFFVAvailable SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGV13 (CK2alpha K68M, CK2beta)
Plasmid#27094DepositorUseTetracycline-regulated expressionTagsHA on CK2alpha and Myc on CK2betaExpressionMammalianMutationK68M on CK2alphaPromoterbidirectional tet-responsive promoter and bidirec…Available SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGV13 (CK2alpha K68M, CK2beta)
Plasmid#27094DepositorUseTetracycline-regulated expressionTagsHA on CK2alpha and Myc on CK2betaExpressionMammalianMutationK68M on CK2alphaPromoterbidirectional tet-responsive promoter and bidirec…Available SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only