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Plasmid#111822PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 294-314
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTK049
Plasmid#171321PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertmCherry fluorescent reporter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_NANOG
Plasmid#64153PurposePhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1_NANOG (NANOG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA4_NANOG
Plasmid#64156PurposePhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA4_NANOG (NANOG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_NANOG
Plasmid#64155PurposePhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA3_NANOG (NANOG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA2_NANOG
Plasmid#64154PurposePhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA2_NANOG (NANOG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pinducer 20 DN-KASHΔPPPL
Plasmid#129280PurposeTet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPLDepositorInsertSYNE1 (SYNE1 Human)
UseLentiviralTagsmcherryExpressionMutationthe last 4 amino acids (PPPL) of the KASH domain …PromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX458-Dual-Guide-Donor-ShuttleOnly
Plasmid#171098PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationXbaI site added upstream of U6 promotor. GtoC mu…PromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-Gbp6-TM-Che
Plasmid#180022PurposeMembrane-bound anti-GFP nanobody conjugated to mCherryDepositorInsertGpb6 nanobody
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)
Plasmid#165026PurposeExpress cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFPDepositorInsertLaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)
Plasmid#165028PurposeExpress cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFPDepositorInsertLaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A-APEX2-ChBP
Plasmid#108879PurposeExpression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)DepositorInsertEGFP-P2A-APEX2-ChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
IDP456
Plasmid#87002Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertbarcode landing site-mCherry-4xboxB
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LT2_14_GlyOx
Plasmid#177711PurposePlasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
pTRX2_5xUAS-ymYPET-tCYC1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sico-Red
Plasmid#84882PurposeCre-inducible shRNADepositorInsertSico-Red
UseAAVTagsmCherryExpressionMutationPromoterEF1a and CMVAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A-APEX2-csChBP
Plasmid#108877PurposeExpression of APEX2 fused to a conditionally stable nanobody against mCherry (includes green cytoplasmic reporter)DepositorInsertEGFP-P2A-APEX2-csChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMET17p-CCG 316
Plasmid#131166PurposeFor ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutantDepositorTypeEmpty backboneUseTagsCCGExpressionYeastMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-AncBE4max
Plasmid#174164PurposeThis is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.DepositorInsertAncBE4max
UseLentiviralTagsP2A-mCherryExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
FOR human NANOS3 TALEN
Plasmid#59722PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertHD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
UseTALENTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
REV human NANOS3 TALEN
Plasmid#59723PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertNG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
UseTALENTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only