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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,551 results for: pcas9

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Showing: 1521 - 1540 of 2551 results

  1. pCMV-T7-ABEmax-SpG

    Plasmid
    #195278
    Purpose
    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFP
    Depositor
    Carsten Lederer, Petros Patsali
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195278-plasmid-map-sequence-id-394868

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  2. HiFi iFE

    Plasmid
    #190141
    Purpose
    Expresses HiFiCas9-POLB (HiFi iFE)
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    HiFi Cas9-POLB
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    SpCas9 R691A mutant, Codon Optimized for mammalia…
    Promoter
    CBh
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190141-plasmid-map-sequence-id-377886

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  5. pX330-MM002-ZFP143-C-terminal-sgRNA

    Plasmid
    #212706
    Purpose
    Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus
    Depositor
    Elzo de Wit
    Insert
    ZFP143 C-terminal sgRNA (Zfp143 Mouse)
    Use
    CRISPR
    Available Since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    212706-plasmid-map-sequence-id-438797

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  6. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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  7. pNSU299_RPL39

    Plasmid
    #166078
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166078-plasmid-map-sequence-id-330349

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  8. pX330.puro

    Plasmid
    #110403
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110403-plasmid-map-sequence-id-213570

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  9. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available Since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161817-plasmid-map-sequence-id-320651

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  11. DBJS-p2.11

    Plasmid
    #246891
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid encoding guide for G3BP1 Nterm.
    Depositor
    David Bartel
    Insert
    G3BP1 Nterm Guide RNA 1 (G3BP1 Human)
    Expression
    Mammalian
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246891-plasmid-map-sequence-id-490580

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  12. DBJS-p2.13

    Plasmid
    #246892
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid encoding guide for G3BP2 Nterm.
    Depositor
    David Bartel
    Insert
    G3BP2 Nterm Guide RNA 1 (G3BP2 Human)
    Expression
    Mammalian
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246892-plasmid-map-sequence-id-490577

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  13. pX330.orup

    Plasmid
    #110404
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110404-plasmid-map-sequence-id-307700

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  15. PX458-AAVS1

    Plasmid
    #113194
    Purpose
    Encodes gRNA for human AAVS1
    Depositor
    Adam Karpf
    Insert
    AAVS1 (PPP1R12C, AAVS1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113194-plasmid-map-sequence-id-216314

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  19. DBJS-p2.16

    Plasmid
    #246893
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid encoding guide for CAPRIN1 Nterm.
    Depositor
    David Bartel
    Insert
    Caprin1 Nterm Guide RNA 1 (CAPRIN1 Human)
    Expression
    Mammalian
    Available Since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246893-plasmid-map-sequence-id-490578

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Showing: 1521 - 1540 of 2551 results