Addgene

We narrowed to 1,639 results for: plasmids pcas

Showing: 1521 - 1540 of 1639 results

Results per page:

Sort results by:

pX459-sgRNA3-RPB1-N-term

Plasmid

#195112

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-sgRNA1-RPB1-N-term

Plasmid

#195110

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

Plasmid

#159658

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

Plasmid

#159657

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
IL1RN gRNA3

Plasmid

#113132

Purpose

Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTij_sg_mmu_Med1_N_terminal

Plasmid

#197870

Purpose

CRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

CRSP210 (Med1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

Plasmid

#159664

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

Plasmid

#159663

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

Plasmid

#159662

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

Plasmid

#159656

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LMX1B-gRNA-C-del

Plasmid

#131516

Purpose

gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

LMX1B-gRNA-C-del

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only
IL1RN gRNA4

Plasmid

#113133

Purpose

Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, Synthetic Biology, and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
IL1RN gRNA2

Plasmid

#113131

Purpose

Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

Plasmid

#83919

Purpose

Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

DsRed-Express2
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Expression

Mutation

Promoter

hUbC

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt

Plasmid

#175563

Purpose

pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

GOXL1

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

RUBY

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

Plasmid

#104563

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1 (D832A)

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGreenII 0229/ProRUBY:GOXL6-Citrine:NOSt

Plasmid

#175568

Purpose

pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-6

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

GOXL6

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

RUBY

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGreenII 0229/ProRUBY:GOXL5-Citrine:NOSt

Plasmid

#175567

Purpose

pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-5

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

GOXL5

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

RUBY

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGreenII 0229/ProRUBY:GOXL4-Citrine:NOSt

Plasmid

#175566

Purpose

pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-4

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

GOXL4

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

RUBY

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGreenII 0229/ProRUBY:GOXL3-Citrine:NOSt

Plasmid

#175565

Purpose

pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-3

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

GOXL3

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

RUBY

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge