We narrowed to 3,131 results for: actin

1. doraWT_rescue_construct

   Plasmid

   #190609

   Purpose

   Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Dorado (CG34401 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-nucGFP11-3xControlgRNA

   Plasmid

   #224568

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBa.GFP-KIF13B

   Plasmid

   #134626

   Purpose

   Mammalian expression of mouse KIF13B 442-1843

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF13B (Kif13b Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Chicken Beta actin

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-mBAF45a IRES GFP

   Plasmid

   #17880

   Depositor

   Jerry Crabtree

   Article

   Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

   Insert

   BAF45a (Phf10 Mouse)
   

   Use

   Tags

   FLAG and IRES EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-Lifeact_Halo_T2A_EGFP

   Plasmid

   #135445

   Purpose

   Mamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

   Insert

   Lifact-HaloTag7 and EGFP
   

   Use

   Tags

   Lifact

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBa.Kif5c 1-559.GFP

   Plasmid

   #45059

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

   Insert

   Kif5c (Kif5c Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   motor domain aa1-559

   Promoter

   Chicken Beta Actin

   Available since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBa.Kif1a 1-396.GFP

   Plasmid

   #45058

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

   Insert

   Kif 1a (Kif1a Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   motor domain aa 1-396

   Promoter

   Chicken Beta Actin

   Available since

   Oct. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

8. mEos2-Fascin-C-10

   Plasmid

   #57376

   Purpose

   Localization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   Fascin (FSCN1 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pLifeAct-miRFP713

   Plasmid

   #136562

   Purpose

   F-actin labeling with a near-infrared fluorescent protein miRFP713

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   LifeAct-miRFP713
   

   Use

   Tags

   miRFP713

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAct:dCas9

    Plasmid

    #78903

    Purpose

    Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mRuby2-Lifeact-7

    Plasmid

    #54674

    Purpose

    Localization: Actin, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Tags

    LifeAct-mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pMADM-beta

    Plasmid

    #36891

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    Beta-Geo
    thymidine kinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLifeAct_mScarlet-H_N1

    Plasmid

    #85055

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAC-dCLK

    Plasmid

    #33153

    Depositor

    Michael Rosbash

    Article

    McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

    Insert

    CLK (Clk Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Mutation

    Promoter

    Actin 5C

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-VinculinI997A_TurboID_Venus

    Plasmid

    #200274

    Purpose

    Expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

    Depositor

    Brenton Hoffman

    Article

    Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

    Insert

    VinculinI997A_TurboID_Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    change isoleucine to alanine at 997 of vinculin

    Promoter

    T7

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-Bgh-GFP-Farpl

    Plasmid

    #115501

    Purpose

    expression of rat Farp1

    Depositor

    Thomas Biederer

    Article

    Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

    Insert

    Farp1 (Farp1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin

    Available since

    Dec. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Clover-Lifeact-7

    Plasmid

    #54551

    Purpose

    Localization: Actin, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. LR (bact: HyPer)

    Plasmid

    #162069

    Purpose

    Used for the diffuse visualization of H2O2 production in cells.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

    Insert

    beta actin 2 (actb2 Zebrafish)
    

    Use

    Gateway donor vector

    Tags

    HyPer

    Expression

    Mutation

    Promoter

    T3

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p35S::mCh-HA-P2A-lifeact-mCh

    Plasmid

    #135234

    Purpose

    constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    mCh-HA-P2A-lifeact-mCh
    

    Use

    Tags

    HA, lifeact, and p2a

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. miniSOG-Lifeact-7

    Plasmid

    #54823

    Purpose

    Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    Lifeact and miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits