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OA-1093B

Plasmid

#194003

Purpose

pBac-U6-crCHIKV-array-UTR-3xp3-tdTomato

Depositor

Omar Akbari

Article

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

4 gRNAs targeting CHIKV

Expression

Insect

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1085F

Plasmid

#191375

Purpose

pBac-U6-crYellow-array-UTR-3xp3-tdTomato

Depositor

Omar Akbari

Article

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

4 gRNAs targeting yellow

Expression

Insect

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Cquin_728

Plasmid

#176656

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Agam_557

Plasmid

#176667

Purpose

Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aaeg_972

Plasmid

#176674

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Agam_557

Plasmid

#176653

Purpose

Expression of sgRNA under An. gambiae U6-1 (AGAP013557) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aalb_726

Plasmid

#176675

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Aaeg_763

Plasmid

#176672

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Aaeg_972

Plasmid

#176660

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL028972) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Cquin_801

Plasmid

#176657

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4B-Cquin_801

Plasmid

#176671

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4B-Cquin_728

Plasmid

#176670

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039728) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTGV-Express SMR5448

Plasmid

#28031

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Expression

Bacterial

Available Since

May 18, 2011

Availability

Academic Institutions and Nonprofits only
pTGV-Light SMR5452

Plasmid

#28032

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Expression

Bacterial

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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pTGV-CAT SMR5453

Plasmid

#28033

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Expression

Bacterial

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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RecA-Ts

Plasmid

#249731

Purpose

RecA plasmid for BAC modification

Depositor

Nathaniel Heintz

Article

Gong et al Nat Protoc. 2010 Sep;5(10):1678-96. doi:

Insert

RecA

Expression

Bacterial

Promoter

RecA promoter

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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OA-1163A

Plasmid

#194001

Purpose

pBac-AedesUbiq-RfxCas13d (Codon optimized)-p10-Opie2-dsRed.

Depositor

Omar Akbari

Article

Dalla Benetta et al CRISPR J. 2023 Dec;6(6):543-556. doi: 10

Insert

Uniq-CasRx (Codon optimized)

Expression

Insect

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-RBM11

Plasmid

#192748

Purpose

Gateway entry vector encoding human RBM11

Depositor

Summer Thyme

Insert

RBM11 (RBM11 Human)

Use

Gateway Cloning

Promoter

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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AAEL017414 TK R

Plasmid

#115396

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

phenoloxidase 2

Expression

Mammalian

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCVD30

Plasmid

#63691

Purpose

pCVD25 with the Tcr gene removed, XbaI is intact, 540 bp of CT A is deleted. Cloned from V. cholerae 395 chromosome.

Depositor

James Kaper

Article

Kaper et al Nature Biotechnology 2, 345 - 349 (1984)

Insert

V. cholera chromosomal DNA

Expression

Bacterial

Mutation

540 bp of CT A is deleted

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
pAC-U63-QtgRNA2.1-tubBFP(HA)

Plasmid

#250794

Purpose

Drosophila transgenic vector: nMAGIC vector with tub promoter, 3x HA tags, and mTagBFP2. Optimized nMAGIC vector with BFP marker.

Depositor

Chun Han

Article

Shen et al Elife. 2026 Mar 11;14:RP108453. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pDnaN-sfGFP

Plasmid

#207246

Purpose

Integrative plasmid for expressing DnaN-sfGFP in Caulobacter crescentus

Depositor

Suliana Manley

Article

Zhang et al Nat Commun. 2024 Apr 24;15(1):3460. doi:

Insert

DnaN(3' end)-sfGFP

Tags

sfGFP

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Pxyl-rsaA

Plasmid

#207250

Purpose

Integrative plasmid for expressing rsaA gene (incl. its promoter) in Caulobacter crescentus at native Pxyl promoter region

Depositor

Suliana Manley

Article

Zhang et al Nat Commun. 2024 Apr 24;15(1):3460. doi:

Insert

rsaA (promoter to codon sequence)

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-mis18a

Plasmid

#192747

Purpose

Gateway entry vector encoding zebrafish mis18a

Depositor

Summer Thyme

Insert

mis18a (si:dkey-189i22.1 Zebrafish)

Use

Gateway Cloning

Promoter

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-sh3bgr

Plasmid

#192727

Purpose

Gateway entry vector encoding zebrafish sh3bgr

Depositor

Summer Thyme

Insert

sh3bgr (zgc:100896 Zebrafish)

Use

Gateway Cloning

Promoter

None

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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AByH

Plasmid

#164152

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AByD

Plasmid

#164150

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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AByC

Plasmid

#164149

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AByB

Plasmid

#164148

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AByA

Plasmid

#163749

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge