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pNB763

Plasmid

#60158

Purpose

Firefly luciferase (Promega) driven by LYS9 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LYS9pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pFGL1252

Plasmid

#118991

Purpose

A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 68bp MCS (multiple cloning site) which contains 12 unique restriction sites.

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Type

Empty backbone

Use

Agrobacterium tumerfaciens-mediated transformation

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL1344

Plasmid

#116902

Purpose

Alp6:Bar:mCherry (SPB/MTOC marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

Alp6 (partial ORF)
Alp6 downstream region

Use

Fungal expression (in magnaporthe oryzae)

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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-226bp goosecoid luciferase

Plasmid

#17158

Depositor

Ken Cho

Article

Watabe et al Genes Dev. 1995 Dec 15. 9(24):3038-50.

Insert

goosecoid promoter (gsc-a Frog)

Use

Luciferase

Tags

luciferase

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pRmHa3-dKDM4A-HA2FL2

Plasmid

#20232

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG2 and HA2

Expression

Insect

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pBacPAK-HFT-dKDM4A

Plasmid

#20233

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG, His, and TEV

Expression

Insect

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
pc3XB-ZF108

Plasmid

#13158

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF108

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pSMT3-fecB

Plasmid

#240158

Purpose

expresses FecB of M. marinum in mycobacteria

Depositor

Wilbert Bitter

Article

Lissner et al mBio. 2025 Dec 12:e0211925. doi: 10.1128

Insert

fecB (MMAR_1650)

Expression

Bacterial

Promoter

HSP60

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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L3928 Filler DNA

Plasmid

#244193

Purpose

Empty vector; modified pcDNA with CMV promoter and bGHpolyA removed to reduce burden

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-SYN1>Fas-Myc/Kir2.1*(E224G Y242F):P2A :dTomato

Plasmid

#242233

Purpose

Express Kir 2.1 and dTomato in the neurons

Depositor

Qingchun Tong

Article

Li et al Cell Rep. 2025 Jun 19;44(7):115863. doi:

Inserts

Fas
Myc/Kir2.1 (E224GY242F)

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

SYN

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE2.1

Plasmid

#235053

Purpose

Expresses Tad-CBE2.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE2.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE2.5

Plasmid

#235054

Purpose

Expresses Tad-CABE2.5 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE2.5-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.1

Plasmid

#235056

Purpose

Expresses Tad-CBE3.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE3.2

Plasmid

#235057

Purpose

Expresses Tad-CBE3.2 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE3.2-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE1.1

Plasmid

#235051

Purpose

Expresses Tad-CABE1.1 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE1.1-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CABE1.2

Plasmid

#235052

Purpose

Expresses Tad-CABE1.2 in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CABE1.2-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SV40-puro-EF1a-HA-NLGN1dAChE-bc

Plasmid

#220437

Purpose

Expresses HA-tagged human NLGN1 with K578A/V579A substitutions

Depositor

Paul Blainey

Article

Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

Insert

Neuroligin-1 (NLGN1 Human)

Use

Lentiviral

Tags

HA-tag

Expression

Mammalian

Mutation

K578A and V579A substitutions

Promoter

EF1a

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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PrecA-CFP--Pconst-YFP

Plasmid

#228099

Purpose

recA promoter driving expression of CFP reporter and constitutive promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

recA & constitutive

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Pconst-CFP--Pconst-YFP

Plasmid

#228100

Purpose

constitutive promoter driving expression of CFP reporter and constitutive promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

constitutive & constitutive

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Pconst-CFP--PrecA-YFP

Plasmid

#228101

Purpose

constitutive promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

constitutive & recA

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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