We narrowed to 3,091 results for: FRI

1. pTTCC

   Plasmid

   #164264

   Purpose

   Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

   Depositor

   Gudmundur Hreggvidsson

   Article

   Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

   Inserts

   Cas9
   gRNA scaffold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pcel and trc

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HiBiT-FZD4

   Plasmid

   #195842

   Purpose

   Expresses human FZD4 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD4 (FZD4 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

   Plasmid

   #193942

   Purpose

   a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.

   Depositor

   Greg Delgoffe

   Article

   Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122

   Insert

   heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HiBiT-FZD2

   Plasmid

   #195840

   Purpose

   Expresses human FZD2 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD2 (FZD2 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-fDIO-Cre

   Plasmid

   #121675

   Purpose

   Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

   Depositor

   Esteban Engel, Alexander Nectow

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Has Service

   AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

   Insert

   NLS-CRE-HA
   

   Use

   AAV

   Tags

   3xHA and SV40-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherryC2-Gephyrin P1

   Plasmid

   #68820

   Purpose

   mCherry tagged Geph for mammalian expression

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyrin…

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMVp-dCas9-3xNLS-VP64 (Construct 1)

   Plasmid

   #55195

   Purpose

   Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/hUBC

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX311-Cas9

   Plasmid

   #118018

   Purpose

   Constitutive expression of Cas9

   Depositor

   David Root, William Hahn

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ORAI1-YFP L273D-L276D

   Plasmid

   #114182

   Purpose

   ORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.

   Depositor

   Nicolas Demaurex

   Article

   Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

   Insert

   ORAI1-YFP L273D-L276D (ORAI1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   L273D/L276D

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ORAI1-YFP V107M

    Plasmid

    #114180

    Purpose

    ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).

    Depositor

    Nicolas Demaurex

    Article

    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

    Insert

    ORAI1-YFP V107M (ORAI1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    V107M

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-NLS-actin

    Plasmid

    #118380

    Purpose

    Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Tags

    Expression

    Mutation

    NLS

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-TRE3G-NFIA+SOX9

    Plasmid

    #129455

    Purpose

    Inducible overexpression of NFIA and Sox9 in human cells.

    Depositor

    Su-Chun Zhang

    Article

    Li et al Stem Cell Reports. 2018 Oct 9;11(4):998-

    Insert

    Sox 9 (SOX9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFPC2-CB2 SH3-

    Plasmid

    #68822

    Purpose

    EGFP tagged Collybistin expression in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

    Insert

    Collybistin (Arhgef9 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCOTS-pyl-GFP(35TAG)

    Plasmid

    #92047

    Purpose

    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Replicative expression plasmid for cyanobacteria …

    Tags

    Histag

    Expression

    Mutation

    Tyrosine in position 35 of the GFP was mutated f…

    Promoter

    LeuP (native S. elongatus promoter), PpsbII, and …

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. HiBiT-FZD6

    Plasmid

    #195844

    Purpose

    Expresses human FZD6 with an N-terminal HiBiT; contains M345L

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD6 (FZD6 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    M345L

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hsyn-flex-Voltron

    Plasmid

    #119035

    Purpose

    Cre-dependant expression of Voltron in neurons

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hsyn

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICE-HA-Ku70-siR-WT

    Plasmid

    #82329

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

    Insert

    Ku70 (XRCC6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tet

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. 6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet

    Plasmid

    #216386

    Purpose

    Bacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Dishevelled-2 (Dvl2 Mouse)
    

    Use

    Tags

    6xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet

    Plasmid

    #216387

    Purpose

    Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Dishevelled-2 (Dvl2 Mouse)
    

    Use

    Tags

    6xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.E2.ChETA

    Plasmid

    #220310

    Purpose

    Directs ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.

    Depositor

    Matt Rowan

    Article

    Kumar et al Nat Commun. 2024 Apr 1;15(1):2823. doi:

    Insert

    ChETA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    S5E2

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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