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Plasmid#176178Purposeyeast MoClo level-0 part plasmid type 6 containing non-coding DNA spacer for construction of MoClo plasmids without yeast markerDepositorInsert41 bp non-coding DNA spacer from pYTK048
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTH32 [pmyo-3::YFP-CaCyclOp(A-2x)::SL2::mCherry]
Plasmid#168168PurposeExpression of YFP-CaCyclOp(A-2x) in BWMs of C. elegansDepositorInsertYFP-CaCyclOp(A-2x)
UseTagsYFPExpressionWormMutationE497K and C566DPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH42 [punc-17::YFP-CaCyclOp(A-2x)::SL2::mCherry]
Plasmid#168171PurposeExpression of YFP-CaCyclOp(A-2x) in cholinergic motor neurons of C. elegansDepositorInsertYFP-CaCyclOp(A-2x)
UseTagsYFPExpressionWormMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG
Plasmid#158132PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the first serine residue…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG2
Plasmid#158134PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the second serine residu…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG3
Plasmid#158136PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the third alanine residu…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-altTAG4
Plasmid#158137PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)DepositorInsertBFP-GFP fragment
UseTagsExpressionYeastMutationCloned in a TAG codon at the third serine residue…PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG
Plasmid#130684PurposeExpresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationD453_F489del, E653_G1960delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG
Plasmid#130683PurposeExpresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationD409_E452del, E653_G1960delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4A RNAiR
Plasmid#115172Purposeexpresses RNAi-resistant Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
UseTagsmycExpressionMammalianMutationSilent mutation between amino acids in ORF (401-4…PromoterCMVAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTIM
Plasmid#81198PurposepTIM in vitro transcription backbone plasmid with two multiple cloning sites and two Rho-independent transcriptional terminators.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab8 DN
Plasmid#58451Purposeexpression of drosophila YFP Rab8 dominant negative mutantDepositorInsertRab8 (Rab8 Fly)
UseTagsYFPExpressionInsectMutationT22N (dominant negative)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 DN
Plasmid#58445Purposeexpression of drosophila YFP-Rab2 dominant negative mutantDepositorInsertRab2 (Rab2 Fly)
UseTagsYFPExpressionInsectMutationS20N (dominant negative)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab2 CA
Plasmid#58443Purposeexpression of drosophila YFP-Rab2 constitutively active mutantDepositorInsertRab2 (Rab2 Fly)
UseTagsYFPExpressionInsectMutationQ65L (constitutive active)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab14 DN
Plasmid#37715DepositorInsertRab14 (Rab14 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS49N (dominant negative)PromoterGAL4Available sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-mnx1:ReaChR-citrine
Plasmid#169046PurposeDestination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.DepositorInsertReaChR-Citrine
UseTagsCitrineExpressionMammalianMutationPromoter3xMnx1Available sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE NFkB-TA-LUC-UBC-GFP-W
Plasmid#49343PurposepHAGE NFkB-TA-LUC-UBC-GFP-WDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pPV-EF1a-mRxxFP1(AfeI)-iP-A
Plasmid#204625PurposeCloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Pmel-1_TCRB
Plasmid#175288PurposeExpresses Pmel-1 TCR beta chainDepositorInsertT cell receptor beta chain (Tcrb Mouse)
UseSuitable for ivtTagsExpressionMammalianMutationPromoterCMV and T7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only