We narrowed to 4,258 results for: biorxiv

1. pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

   Plasmid

   #218636

   Purpose

   Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Lentiviral

   Tags

   APEX2-3xFLAG

   Expression

   Mammalian

   Mutation

   H781E, Q782R, F785A

   Promoter

   UBC

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

   Plasmid

   #218635

   Purpose

   Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Lentiviral

   Tags

   APEX2-3xFLAG

   Expression

   Mammalian

   Mutation

   N1008R, R1009E, E1010R, R1013E

   Promoter

   UBC

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. GOLIM4 g2 LentiCRISPRv2-mCherry

   Plasmid

   #218663

   Purpose

   Knockout vector for human GOLIM4 (GPP130/GOLPH4)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   GOLIM4 (GOLIM4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. GOLIM4 g1 LentiCRISPRv2-mCherry

   Plasmid

   #218662

   Purpose

   Knockout vector for human GOLIM4 (GPP130/GOLPH4)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   GOLIM4 (GOLIM4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-SFFVp-gI-BlastR

   Plasmid

   #207179

   Purpose

   Gateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastR

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFVp

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SV40p-gI-BlastR

   Plasmid

   #207182

   Purpose

   Gateway compatible Lentiviral Expression Plasmid SV40p-IRES-BlastR

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1_NAP1-eGFP

   Plasmid

   #216837

   Purpose

   For mammalian expression of NAP1-GFP (Internal ref. SMC1638)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Efg1-ΔDBD-Q443P

   Plasmid

   #216401

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-Q443P
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Glutamine 443 to Proline, and deleted ami…

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-MBP-TEVpCS-ADGRE4 CTFdeltaTA-FLAG

   Plasmid

   #217725

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE4
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-179

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

    Plasmid

    #215000

    Purpose

    Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)
    

    Use

    Hdr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-6xHis-TrxF

    Plasmid

    #207454

    Purpose

    E. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tag

    Depositor

    Joseph Jez, Sarah Stainbrook

    Article

    Stainbrook et al bioRxiv 2022.08.24.505184

    Insert

    Thioredoxin F
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    T7

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210749

    Purpose

    Coding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    CjSp D8A Cas9
    Bilbo sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D8A, N-terminal Cj Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH1-CMV-FIP200-V44D/V314D-mCherry-Hygro

    Plasmid

    #210855

    Purpose

    Lentiviral expression vector encoding FIP200 mutant-mCherry

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    V44D/V314D

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH1-CMV-FIP200-V314D-mCherry-Hygro

    Plasmid

    #210854

    Purpose

    Lentiviral expression vector encoding FIP200 mutant-mCherry

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    V314D

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDH1-CMV-FIP200-V44D-mCherry-Hygro

    Plasmid

    #210853

    Purpose

    Lentiviral expression vector encoding FIP200 mutant-mCherry

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    V44D

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-TSF-ATG101

    Plasmid

    #210858

    Purpose

    Mammalian expression of ATG101

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG101 (ATG101 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF

    Plasmid

    #210842

    Purpose

    Mammalian expression of FIP200 NTD mutant

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L324D/R543D/R548D

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR4 AvrRpm1

    Plasmid

    #209185

    Purpose

    pENTR4 plasmid with CDS of Pseudomonas syringae pv maculicola type III effector AvrRpm1 without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    AvrRpm1
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL1_38

    Plasmid

    #199045

    Purpose

    Level 1 plasmid, barcoded ORI

    Depositor

    Nili Ostrov, Cultivarium Tools, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pIJ101 ORI plus NGS barcode 15
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL0_18 [AMA1 long ORI]

    Plasmid

    #198931

    Purpose

    Level 0 part

    Depositor

    Nili Ostrov, Cultivarium Tools, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    AMA1 long ORI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge