We narrowed to 2,799 results for: fas

1. pFS462

   Plasmid

   #89065

   Purpose

   his7 integration plasmid with Z3EVpr:GFP

   Depositor

   Nick Rhind

   Article

   Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

   Insert

   Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
   

   Use

   S. pombe his7 integration vector

   Promoter

   Z3EV

   Available Since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CaMKIIa-eNpHR-EYFP-WPRE

   Plasmid

   #20946

   Purpose

   3rd gen lentiviral expression of halorhodopsin 2.0 (eNpHR) fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   halorhodopsin
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO_NES_STf

   Plasmid

   #182001

   Purpose

   CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   NES-STf
   

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE1108

   Plasmid

   #202772

   Purpose

   CRISPR-KO in Arabidopsis thaliana

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

   Inserts

   FAST marker
   pRPS5a:zCas9_ttriple
   ccdB cassette (BsaI-excisable)
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKL2013

   Plasmid

   #172182

   Purpose

   A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.

   Depositor

   Kan Wang

   Article

   McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d

   Insert

   mCherry
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MMLV-CAG-G_oT7pol-IRES-BSD

   Plasmid

   #172369

   Purpose

   Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   oT7pol + BSD
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-myrSNAP

   Plasmid

   #60098

   Purpose

   Expresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditional

   Depositor

   Gregory Jefferis

   Article

   Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

   Inserts

   SNAP-tag
   histone-GFP
   

   Use

   AAV and Mouse Targeting

   Expression

   Mammalian

   Available Since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. eGFP-CBP

   Plasmid

   #169211

   Purpose

   Expression of eGFP-CBP protein for chitin visualization

   Depositor

   James S Fraser

   Article

   Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

   Insert

   Chitinase A1 Chitin Binding Domain
   

   Tags

   6xHis, Mxe Gyr Intein, and eGFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pExpreS2-1

   Plasmid

   #175360

   Purpose

   ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

   Depositor

   ExpreS2ion Biotechnologies

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSG5-hSyntenin2a

    Plasmid

    #89432

    Purpose

    mammalian expression of human Syntenin2a

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCoofy48

    Plasmid

    #55186

    Purpose

    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC1750

    Plasmid

    #127984

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO_LAMP1_STf

    Plasmid

    #182006

    Purpose

    CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    LAMP1_STf
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mTubulin NLS-mCherry-LEXY

    Plasmid

    #182597

    Purpose

    Drosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    mCherry
    

    Tags

    LEXY and NLS

    Expression

    Insect

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-NBid-PhoCl1-CBid

    Plasmid

    #164050

    Purpose

    Optogenetic manipulation of cell apoptosis using PhoCl1

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-PhoCl1-CBid
    

    Tags

    kozak sequence

    Expression

    Mammalian

    Promoter

    CMV promotor

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO_Lifeact_STf

    Plasmid

    #182011

    Purpose

    CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    Lifeact_STf
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-myrSNAP-CON

    Plasmid

    #60099

    Purpose

    Expresses membrane-targeted SNAP(f)-tag and histone-GFP, conditional

    Depositor

    Gregory Jefferis

    Article

    Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

    Inserts

    SNAP-tag
    histone-GFP
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-hSyntenin2a

    Plasmid

    #89436

    Purpose

    mammalian expression of EGFP tagged human Syntenin2a

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP2 (SDCBP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUPD2+pCmYLCV911

    Plasmid

    #170878

    Purpose

    Phytobrick - pCmYLCV911

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    pCmYLCV911
    

    Expression

    Plant

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFBCPDL6

    Plasmid

    #67285

    Purpose

    CPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expression

    Depositor

    Aziz Sancar

    Article

    Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.

    Insert

    CPD photolyase (phr Fly)
    

    Use

    Bacterial vector, insert not expressed

    Tags

    Flag

    Mutation

    V275E*

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only