We narrowed to 2,612 results for: ubiquitin

1. GFP-ataxin-3 C-t

   Plasmid

   #89981

   Purpose

   Expresses the GFP-tagged C-terminus of ataxin-3

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-182

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GP805

   Plasmid

   #164545

   Purpose

   Expresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

   Depositor

   Gregory Pazour

   Article

   Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   Smoothened K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   All 21 cytoplamic Lysines of Smoothened changed t…

   Promoter

   GgCryD1

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pfast bac cry1myc

   Plasmid

   #51892

   Purpose

   Backbone plasmid is pfastBacHTa from invitrogen

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   cry1 (Cry1 Mouse)
   

   Use

   Tags

   3x Flag and myc

   Expression

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pGEX-6P-1 p37 SHPmut

   Plasmid

   #113503

   Purpose

   Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p37 (Ubxn2b Mouse)
   

   Use

   Tags

   GST-PreScission protease site

   Expression

   Bacterial

   Mutation

   F215A/E218A/QKL220-222AAA

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)

   Plasmid

   #119312

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Only AA 1-500

   Promoter

   CMV

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PHAGE-P CMVt N-HA hE6-AP I (AA 1-376)

   Plasmid

   #119311

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I AA 1-376 in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Only AA 1-376

   Promoter

   CMV

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)

   Plasmid

   #119316

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Only AA 481-852

   Promoter

   CMV

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

   Plasmid

   #85821

   Purpose

   iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   Zf Ubi::cMyc oDam_f_GFPoNLS
   

   Use

   Tol2 based vector

   Tags

   cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

   Expression

   Mutation

   L122A. Codon optimization compatible to work in M…

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pfast bac Rbx-HA

   Plasmid

   #51893

   Purpose

   Backbone plasmid is pfastBacHTa from invitrogen

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   Rbx1 (RBX1 Human)
   

   Use

   Tags

   HA

   Expression

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-6P-1 p37 deltaUBX

    Plasmid

    #113504

    Purpose

    Bacterial expression of GST-tagged p37 lacking the UBX domain

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 251-331 (stop codon behind AA250)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFPc1 K-RAS 4A

    Plasmid

    #236451

    Purpose

    transient overexpression of K-RAS 4A in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

    Insert

    K-RAS 4A (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1-NEDD4L_ΔC2 (157)

    Plasmid

    #187720

    Purpose

    Express GST-tagged NEDD4L ΔC2 (157) in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 157-975 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Insect

    Mutation

    deletion of residues 1-156

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBacHTA-NEDD4L_FL

    Plasmid

    #187722

    Purpose

    Express His-tagged NEDD4L FL in insect cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL (NEDD4L Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a(+)-NEDD4L_FL

    Plasmid

    #187705

    Purpose

    Express his-tagged NEDD4L FL in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a(+)-NEDD4L_W4-HECT

    Plasmid

    #187710

    Purpose

    Express his-tagged NEDD4L W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 543-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-542

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-NEDD4L_FL

    Plasmid

    #187712

    Purpose

    Express GST-tagged NEDD4L FL in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1-NEDD4L_WW34

    Plasmid

    #187718

    Purpose

    Express GST-tagged NEDD4L WW34 tandem domains in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-581 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    only residues 492-581

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX6P1-NEDD4L_HECT

    Plasmid

    #187719

    Purpose

    Express GST-tagged NEDD4L HECT domain in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 594-975 (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-593

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmch-mTet1-ZF-CD pc3956

    Plasmid

    #201709

    Purpose

    Expresses of mcherry-tagged mTet1-ZF-CD in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1-ZF_CD (Tet1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Zinc finger domain of Tet1 fused to its catalytic…

    Promoter

    CAG

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETDUET-GST AO7RE ∆238-245

    Plasmid

    #139318

    Purpose

    GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    AO7 cloned into the first multiple cloning site o…

    Promoter

    T7 promoter-1

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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