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Plasmid#195369PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6DepositorInserthA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-ABE5
Plasmid#195371PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8DepositorInsertecTadA8e-(GGGGS)x8-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…Tags5' and 3' NLSExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-ABE8
Plasmid#195569PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3DepositorInsertecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(gsagsaagsgef…Tags5' and 3' NLSExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE2
Plasmid#195368PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTENDepositorInserthA3A/Y130F-XTEN-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-ABE4
Plasmid#195370PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6DepositorInsertecTadA8e-(GGGGS)x6-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…Tags5' and 3' NLSExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE1
Plasmid#195367PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAGDepositorInserthA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-xten plus 3…Tags3xflagExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10878
Plasmid#183964PurposeCAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD156R, E292R, D235R, D350RPromoterCAGAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
plenti-Lb-Cas12a-2xNLS
Plasmid#155046PurposeLentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoterDepositorInsertLbCas12a
UseCRISPR and LentiviralTagsNucleoplasmin NLS, Myc-tag and SV40 NLSExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-STU-Lb
Plasmid#138096PurposeGolden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-STU-Lb
Plasmid#138105PurposeGolden Gate entry vector for 4th crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-STU-Lb
Plasmid#138102PurposeGolden Gate entry vector for 3rd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-STU-Lb
Plasmid#138099PurposeGolden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA-hcr3
Plasmid#193660PurposeExpression of tandem pre-sgRNA array hcr3 for LbCas12aDepositorInsertU6-CFTR-sgRNA-KLF4-sgRNA-TET1-sgRNA-tRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA-hcr5
Plasmid#193661PurposeExpression of tandem pre-sgRNA array hcr5 for LbCas12aDepositorInsertU6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA-pcr4
Plasmid#193662PurposeExpression of tandem pre-sgRNA array pcr4 for LbCas12aDepositorInsertU6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT10446
Plasmid#192353PurposeL0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stopDepositorInsertTTCG_Stop-t35S_CGCT
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGT5439
Plasmid#192351PurposeL0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stopDepositorInsertTTCG_Stop-tOCS_CGCT
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGT9403
Plasmid#192352PurposeL0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stopDepositorInsertTTCG_Stop-tNOS_CGCT
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RR-ABE8e-V106W
Plasmid#193656PurposeExpression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertdLbCas12a-TadA*8e-V106W, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-G532R/K595R, TadA*8e-V106WPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RR-A3A-N57G
Plasmid#193654PurposeExpression of base editor RR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescenceDepositorInsertRR-dLbCas12a-hA3A-N57G, EGFP
UseTagsExpressionMammalianMutationdLbCas12a-G532R/K595R, hAPOBEC3A-N57GPromoterCMVAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only