We narrowed to 246 results for: LbCas12a

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SM420_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-TruSeq_3

Plasmid

#244224

Purpose

Vector for cloning LbCas12a crRNA array libraries. Contains 3' TruSeq sequence for easy NGS library construction

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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SM425_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-KAN-DR

Plasmid

#244225

Purpose

Vector for golden gate cloning LbCas12a crRNA arrays. Contains mCherry-P2A-puroR selectable marker.

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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EC59103

Plasmid

#191785

Purpose

LbCas12a expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas12a+ 8 introns-T-E9

Use

CRISPR

Expression

Plant

Mutation

D156R

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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p33Lb

Plasmid

#158156

Purpose

Expression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL1P1_crU626

Plasmid

#191776

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P2_crU626

Plasmid

#191777

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P7_crU626

Plasmid

#191782

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P6_crU626

Plasmid

#191781

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P3_crU626

Plasmid

#191778

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P4_crU626

Plasmid

#191779

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P5_crU626

Plasmid

#191780

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0038

Plasmid

#117605

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL-his-LbCpf1-EC

Plasmid

#79008

Purpose

Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

LbCpf1

Tags

MBP-6xHis-NLS

Expression

Bacterial

Promoter

tac promoter

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pMB30

Plasmid

#122247

Purpose

Lentivirus delivery for stable expression of Lb crRNA, has LbDR; Cloning vector for expression of LbCas12a crRNA. It contains BsmBI site for cloning.

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE

Plasmid

#140087

Purpose

Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

LbCpf1 (LbCas12a) crRNA backbone

Use

Lentiviral

Tags

EF1a-Puro

Expression

Mammalian

Promoter

U6

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMB34

Plasmid

#122245

Purpose

Lentivirus delivery for stable expression of LbCas12a-VPR

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Promoter

EFs

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2-HybridBarcode

Plasmid

#174037

Purpose

For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

CRISPR target sites

Use

Tol2 transgenesis

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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LbCpf1-2NLS

Plasmid

#102566

Purpose

bacterial expression of LbCpf1-2NLS

Depositor

Jennifer Doudna

Article

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

LbCpf1

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Promoter

T7

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hLbCpf1(TYCV) (pY230)

Plasmid

#89355

Purpose

Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

G532R/K595R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

Plasmid

#140086

Purpose

Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

LbCpf1 (LbCas12a) crRNA backbone

Use

Lentiviral

Tags

EF1a-Puro-T2A-BFP

Expression

Mammalian

Promoter

U6

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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