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Showing: 141 - 157 of 157 results

  1. pAVA-Gal11p-LGF2(fs)

    Plasmid
    #127482
    Purpose
    Negative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interaction
    Depositor
    Joel Malek
    Article
    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1
    Insert
    Gal11p-LGF2(frame shifted) (GAL11 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    LGF2 is frame shifted by the insert of one nucleo…
    Promoter
    Available since
    Nov. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127482-plasmid-map-sequence-id-262919

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  2. pAVA-LGF2(fs)-Gal11p(fs)

    Plasmid
    #127483
    Purpose
    Negative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interaction
    Depositor
    Joel Malek
    Article
    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1
    Insert
    LGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    One nucleotide inserted between lambda CI and LGF…
    Promoter
    Available since
    Nov. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127483-plasmid-map-sequence-id-262924

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  3. AP2u2-CMV-S_protein

    Plasmid
    #226456
    Purpose
    Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Insert
    Spike Protein
    Use
    Tags
    6xHis Tag, Foldon Domain, and Thrombin Cleavage S…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226456-plasmid-map-sequence-id-458388

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  4. AP2u2-CMV-Ace2-IgG1

    Plasmid
    #226455
    Purpose
    Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Inserts
    Angiotensin Converting Enzyme 2
    Immunoglobulin Heavy Constant Gamma 1
    Use
    Tags
    6xHis Tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226455-plasmid-map-sequence-id-458394

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  5. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162877-plasmid-map-sequence-id-321985

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  7. pD649-HAsp-HAVCR2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156584
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    HAVCR2 (HAVCR2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156584-plasmid-map-sequence-id-426824

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  12. pDONR223_HAVCR2_WT_V5

    Plasmid
    #82965
    Purpose
    Gateway Donor vector containing HAVCR2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Jesse Boehm, Matthew Meyerson, David Root
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    HAVCR2 (HAVCR2 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    R140L
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82965-plasmid-map-sequence-id-162701

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  13. HS_NAM-B1

    Plasmid
    #154064
    Purpose
    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    HvHSP17, OsAct, and ZmUbi
    Available since
    Sept. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154064-plasmid-map-sequence-id-313309

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  15. OVC Extended Collection

    Plasmid Kit
    #1000000165
    Purpose
    Four plasmids containing the cloning cassette, eight plasmids containing LSDs engineered to heterodimerize and their binding partners, and five plasmids with different light-activated Opto-caspase 9.
    Depositor
    Harald Janovjak
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 141 - 157 of 157 results