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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,137 results for: sequence

Showing: 141 - 160 of 13137 results

  1. pAG497 mito-iFAST

    Plasmid
    #130819
    Purpose
    Expresses mito-iFAST in mammalian cells (mitochondria labeling)
    Depositor
    Arnaud Gautier
    Insert
    mito-iFAST
    Use
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    130819-plasmid-map-sequence-id-254957

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  2. pCCG_nit9E

    Plasmid
    #228419
    Purpose
    Expression of NIT-9E domain in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of the N terminal G domain and linkage…
    Promoter
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228419-plasmid-map-sequence-id-460292

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  3. pCCG_nit9G

    Plasmid
    #228420
    Purpose
    Expression of NIT-9G domain in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of the C terminal E domain and linkage…
    Promoter
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228420-plasmid-map-sequence-id-460291

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  4. pCCG_nit9_A184-G203

    Plasmid
    #228421
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of A184-G203
    Promoter
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228421-plasmid-map-sequence-id-460290

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  5. pCCG_nit9_G204-G223

    Plasmid
    #228422
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of G204-G223
    Promoter
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228422-plasmid-map-sequence-id-460288

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  6. pCCG_nit9_E224-G243

    Plasmid
    #228423
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of E224-G243
    Promoter
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228423-plasmid-map-sequence-id-460277

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  7. pCCG_nit9_K264-P282

    Plasmid
    #228425
    Purpose
    Expression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of K264-P282
    Promoter
    Available Since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228425-plasmid-map-sequence-id-460271

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  8. pCCG_nit9G_linker

    Plasmid
    #228413
    Purpose
    Expression of NIT-9G with linkage region at the C terminus in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of the C terminal E domain
    Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228413-plasmid-map-sequence-id-459453

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  9. pCCG_linker_nit9E

    Plasmid
    #228412
    Purpose
    Expression of NIT-9E with linkage region at the N terminus in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    truncation of the N terminal G domain
    Promoter
    Available Since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228412-plasmid-map-sequence-id-459452

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  10. UCT1m

    Plasmid
    #121041
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UCT-part guide RNA (Stanley Qi sequence)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121041-plasmid-map-sequence-id-245418

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  11. pBAD-tdTEVDB_C-15A/G4C

    Plasmid
    #177205
    Purpose
    Permuted td intron with evolved initiation sequence for the expression of circular mRNA
    Depositor
    Stephen Fried
    Article
    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175
    Insert
    Permuted GFP with evolved initiation sequence for circular mRNA
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Permuted sfGFP after residue 52 & Changed Cyt…
    Promoter
    araBAD
    Available Since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177205-plasmid-map-sequence-id-373215

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  13. pDS24

    Plasmid
    #58952
    Purpose
    Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
    Depositor
    Thomas Fox
    Article
    Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93
    Insert
    Mitochondrially coded Arg8 protein
    Use
    Tags
    Expression
    Mutation
    Coding sequence synthetic in S. cerevisiae mitoch…
    Promoter
    n/a
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pET151-D-TOPO-PARP14-SΔK

    Plasmid
    #216658
    Purpose
    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.
    Depositor
    Herwig Schüler
    Article
    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.
    Insert
    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
    Use
    Tags
    6xHis-V5-TEV
    Expression
    Bacterial
    Mutation
    Deletion starting from Q1390 to D1448 and substit…
    Promoter
    T7
    Available Since
    Aug. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    216658-plasmid-map-sequence-id-429340

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  18. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid
    #164266
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.
    Depositor
    Nikolaus Rajewsky
    Article
    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    U6 promoter from W05B2.8
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164266-plasmid-map-sequence-id-325780

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  20. pET28-NFMshuf1

    Plasmid
    #232058
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Medium tail domain, charge shuffle 1
    Use
    Tags
    6xHis, thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232058-plasmid-map-sequence-id-464062

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Showing: 141 - 160 of 13137 results