We narrowed to 9,401 results for: Uty

1. pCS2+ cMyc Dam_f_GFPoNLS

   Plasmid

   #85820

   Purpose

   iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   cMyc Dam_f_GFPoNLS
   

   Use

   Tags

   cMyc and oNLS (optimized nuclear localization sig…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A.

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICE-EGFP-FLAG-Ku70siR-Mut6E

   Plasmid

   #46962

   Purpose

   For constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku70 (XRCC6 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Mut6E corresponding to K282E K287E T300E K331E K3…

   Promoter

   CMV Tet

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBT9-Hook2

   Plasmid

   #198524

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   Hook2 (HOOK2 Human)
   

   Use

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   His488Gln substitution

   Promoter

   ADH1

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-HA-Flag-natT1R2 a22-end_S212N

   Plasmid

   #113951

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, S212N substitution

   Promoter

   CMV

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ oDam_f_GFPoNLS

   Plasmid

   #85819

   Purpose

   iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   oDam_f_GFPoNLS
   

   Use

   Tags

   oNLS (optimized nuclear localization signal) C te…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A. Codon optimization compatible to work in M…

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1alpha-soCoChR-GFP

   Plasmid

   #107709

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-GFP
   

   Use

   AAV

   Tags

   EGFP and KA2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG470 LifeAct-iFAST

   Plasmid

   #130821

   Purpose

   Expresses LifeAct-iFAST in mammalian cells (actin labeling)

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   LifeAct-iFAST
   

   Use

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG498 MAP4-iFAST

   Plasmid

   #130820

   Purpose

   Expresses MAP4-iFAST in mammalian cells (microtubule labeling)

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   MAP4-iFAST
   

   Use

   Tags

   Myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p21StBD

   Plasmid

   #190269

   Purpose

   Constitutively expresses a protein of interest fused to a GAL4 DNA-binding domain. Targeted integration to genomic locus YPRC-tau 3, Chromosome XVI.

   Depositor

   Assaf Mosquna

   Article

   Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

   Type

   Empty backbone

   Use

   Synthetic Biology; Yeast genome-integrating vector

   Tags

   GAL4-BD

   Expression

   Mutation

   Promoter

   TDH3

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. attB53_SNCB-_attB53

    Plasmid

    #183615

    Purpose

    RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Inserts

    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    

    Use

    Rmce

    Tags

    Myc and human CD8a signal sequence

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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