Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 3,822 results for: Gla

Showing: 1581 - 1600 of 3822 results

  4. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid
    #140980
    Purpose
    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasS-RLuc8
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140980-plasmid-map-sequence-id-300680

    Enlarge

  5. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Article
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Expression
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80424-plasmid-map-sequence-id-151441

    Enlarge

  6. (383) pcDNA3.1-HA-nBC2-OGA(544-706)

    Plasmid
    #168100
    Purpose
    HA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA
    Depositor
    Christina Woo
    Article
    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/
    Insert
    HA-nBC2-OGA(544-706)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    OGA truncated to contain residues 544-706
    Promoter
    T7/CMV
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    168100-plasmid-map-sequence-id-333446

    Enlarge

  8. (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

    Plasmid
    #168102
    Purpose
    HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA
    Depositor
    Christina Woo
    Article
    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/
    Insert
    HA-nEPEA-(EAAAK)3-OGA(544-706)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    OGA truncated to contain residues 544-706
    Promoter
    T7/CMV
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    168102-plasmid-map-sequence-id-333448

    Enlarge

  13. pETM6-G6-vioABE

    Plasmid
    #73438
    Purpose
    Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PG6 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73438-plasmid-map-sequence-id-135337

    Enlarge

  15. TKTL1

    Plasmid
    #72419
    Purpose
    Study the role of aberrant expression of TKTL1 in HNSCC tumorigenesis
    Depositor
    David Sidransky
    Article
    Sun et al Clin Cancer Res. 2010 Feb 1;16(3):857-66
    Insert
    TKTL1 (TKTL1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    may have one or two synonymous substitutions
    Promoter
    CMV
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid
    #205021
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorT-mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205021-plasmid-map-sequence-id-407999

    Enlarge

  19. pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

    Plasmid
    #198054
    Purpose
    All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning
    Depositor
    Lacra Bintu
    Article
    Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.
    Inserts
    rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
    3xFLAG-insert
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G and pEF
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    198054-plasmid-map-sequence-id-391373

    Enlarge

Showing: 1581 - 1600 of 3822 results