Addgene

We narrowed to 13,155 results for: Mpl;

Showing: 3161 - 3200 of 13155 results

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pRS416-ADH1p- BASU-FKBP-NLS-HA3

Plasmid

#227995

Purpose

Expresses BASU-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGGC018

Plasmid

#228232

Purpose

Level 0 for LhG4 (GreenGate C module)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

GR-LhG4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

-

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-TID-FRB-NLS-HA3

Plasmid

#227997

Purpose

Expresses TurboID-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-TID-FKBP-HA3

Plasmid

#227998

Purpose

Expresses TurboID-FKBP fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-ADH1p-TID-FKBP-NLS-HA3

Plasmid

#227999

Purpose

Expresses TurboID-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-mTID-FRB-HA3

Plasmid

#228000

Purpose

Expresses miniTurboID-FRB fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-ADH1p-mTID-FRB-NLS-HA3

Plasmid

#228001

Purpose

Expresses miniTurboID-FRB-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-ADH1p-mTID-FKBP-HA3

Plasmid

#228002

Purpose

Expresses miniTurboID-FKBP fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-ADH1p-mTID-FKBP-NLS-HA3

Plasmid

#228003

Purpose

Expresses miniTurboID-FKBP-NLS fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-HIS3p-TID-FRB-HA3

Plasmid

#228004

Purpose

Expresses TurboID-FRB fusion from HIS3 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-HIS3p-TID-FRB-NLS-HA3

Plasmid

#228005

Purpose

Expresses TurboID-FRB-NLS fusion from HIS3 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-HIS3p-TID-FKBP-HA3

Plasmid

#228006

Purpose

Expresses TurboID-FKBP fusion from HIS3 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-HIS3p- TID-FKBP-NLS-HA3

Plasmid

#228007

Purpose

Expresses TurboID-FKBP-NLS fusion from HIS3 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-CHA1p-TID-FKBP-HA3

Plasmid

#228014

Purpose

Expresses TurboID-FKBP fusion from CHA1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

CHA1

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-MET25p-TID-FRB-HA3

Plasmid

#228016

Purpose

Expresses TurboID-FRB fusion from MET25 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

MET25

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-MET25p-TID-FRB-NLS-HA3

Plasmid

#228017

Purpose

Expresses TurboID-FRB-NLS fusion from MET25 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

MET25

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-MET25p-TID-FKBP-HA3

Plasmid

#228018

Purpose

Expresses TurboID-FKBP fusion from MET25 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

MET25

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-MET25p- TID-FKBP-NLS-HA3

Plasmid

#228019

Purpose

Expresses TurboID-FKBP-NLS fusion from MET25 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

MET25

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFa6-TID-FKBP-3HA-KanMX6

Plasmid

#228024

Purpose

To integrate TID-FKBP fusion at yeast promoter; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-BASU-HA3-KanMX6

Plasmid

#227978

Purpose

To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-BioID2-HA3-KanMX6

Plasmid

#227976

Purpose

To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID2

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-BirA-HA3-KanMX6

Plasmid

#227974

Purpose

To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDestTol2pA2-5.0dand5:EGFP

Plasmid

#223327

Purpose

Expresses EGFP in Kupffer's vesicle cells in zebrafish embryos

Depositor

Hiroyuki Takeda

Article

Ikeda et al Dev Growth Differ. 2022 Apr;64(3):163-17

Insert

5.0kb dand5 promoter (dand5 Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTE5417_p3WJdB_promoter dropout vector

Plasmid

#221191

Purpose

Promoter probe vector with dropout sequence for promoter sequences with 3WJdB reporter

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Promoter Placeholder/Dropout Vector

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSliceRep_B_miR-196a.M3

Plasmid

#220471

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSliceRep_A_miR-196a.M3

Plasmid

#220467

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSliceRep_B_miR-196a

Plasmid

#220470

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSliceRep_A_miR-7

Plasmid

#220464

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonorTp-fugfp

Plasmid

#211790

Purpose

pDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites

Depositor

Silvia Cardona

Article

Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

Insert

fuGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSliceRep_A_miR-7.M4

Plasmid

#220465

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_VPS26C

Plasmid

#209690

Purpose

Expresses MAC-tagged VPS26C in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS26C (VPS26C Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_VPS35L

Plasmid

#209692

Purpose

Expresses MAC-tagged VPS35L in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

NC_000016.9:g.19710824G>A

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSliceRep_A_miR-196a

Plasmid

#220466

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Minimal CMV

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-UBC_NKX2-1

Plasmid

#221495

Purpose

NKX2-1 short isoform lentiviral overexpression using UBC promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

UBC

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NKX2-1_Long

Plasmid

#221471

Purpose

Gateway cloning of NKX2-1 long isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NKX2-1_W208L

Plasmid

#221474

Purpose

Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

W208L

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_NKX2-1_V205F

Plasmid

#221473

Purpose

Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

V205F

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-EF1a_NKX2-1_V205F

Plasmid

#221485

Purpose

NKX2-1 V205F DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V205F

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3161 - 3200 of 13155 results

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