We narrowed to 12,421 results for: Coli

1. pJK597

   Plasmid

   #71121

   Purpose

   Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

   Depositor

   T. J. Kappock

   Article

   Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

   Insert

   formyl-CoA:oxalate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRAR064

   Plasmid

   #185031

   Purpose

   400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Nucleotides of rpmE 5'UTR scrambled, Only fi…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRAR074-control-sfGFP

   Plasmid

   #185032

   Purpose

   J23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Nucleotides of rpmE 5'UTR scrambled, Only fi…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRAR102

   Plasmid

   #185033

   Purpose

   400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   U54C mutation of rpmE 5'UTR, Only first 90 n…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRAR106

   Plasmid

   #185036

   Purpose

   400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G79U mutation of rpmE 5'UTR, Only first 90 n…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRAR116

   Plasmid

   #185040

   Purpose

   400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   U58C and U70C of rpmE of 5'UTR, Only first 9…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRAR017-L31-sfGFP

   Plasmid

   #185024

   Purpose

   400 nt l31 promoter region, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

   Depositor

   Julius Lucks

   Article

   Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

   Insert

   L31-sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Only first 90 nt of rpmE coding present

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMS22

   Plasmid

   #129710

   Purpose

   Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion

   Depositor

   Alexandra Deaconescu

   Article

   Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

   Insert

   Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
   

   Use

   Tags

   10-Histidine tag cleavable with PreScission Prote…

   Expression

   Bacterial

   Mutation

   RssB E134G:E135S:E136G:E137S

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET His6 GST TEV LIC cloning vector (2G-T)

   Plasmid

   #29707

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, GST, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 MBP TEV LIC cloning vector (2M-T)

    Plasmid

    #29708

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

    Plasmid

    #29722

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mC…

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid

    #86928

    Purpose

    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and td…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

    Plasmid

    #55209

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xAsn, 10xHis, MPB, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHiKdtA

    Plasmid

    #36269

    Depositor

    Christian Raetz

    Article

    Chung et al Biochemistry. 2010 May 18;49(19):4126-37

    Insert

    H. influenzae KdtA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    araC

    Available since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

15. EcAvs4

    Plasmid

    #188794

    Purpose

    Avs4 from Escherichia coli NCTC11132

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    EcAvs4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRKH2

    Plasmid

    #71312

    Purpose

    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

    Depositor

    Todd Klaenhammer, Rodolphe Barrangou

    Article

    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNK045p

    Plasmid

    #156335

    Purpose

    Expresses heterologous rRNA

    Depositor

    Ahmed Badran

    Article

    Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

    Insert

    rRNA operon, orthogonal-antiRBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT antiRBS engineered to orthogonal antiRBS, PS e…

    Promoter

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD24-sfGFPx1

    Plasmid

    #51558

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

    Depositor

    Francisco Malagon, Sankar Adhya

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSM019

    Plasmid

    #128291

    Purpose

    asymmetric plasmid partitioning E. coli - regulatory plasmid

    Depositor

    Matthew Bennett

    Article

    Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

    Insert

    Para - lp-sfyfp-parB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. RepX_pET28a(+)

    Plasmid

    #172199

    Purpose

    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

    Depositor

    Taekjip Ha

    Article

    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

    Insert

    RepX superhelicase
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    C18L, C43S, C167V, S400C, C612A

    Promoter

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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