We narrowed to 3,901 results for: SPL;

1. FLAG-Pol-II 1-22

   Plasmid

   #35178

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal domain repeats 23-52

   Promoter

   CMV

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

2. FLAG-Pol-II 31-52

   Plasmid

   #35179

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal domain repeats 1-30

   Promoter

   CMV

   Available since

   Oct. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

3. SFG.wtCNa_opt.IRES.eGFP

   Plasmid

   #22489

   Depositor

   Martin Pule

   Article

   Brewin et al Blood. 2009 Sep 21. ():.

   Insert

   codon optimised wild type Calcineurin A (PPP3CA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon Optimised wtCNa

   Promoter

   Available since

   Dec. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-LgBiT-MYPT1(1-314)(KAKA)-Flag

   Plasmid

   #224894

   Purpose

   Mammalian expression of LgBiT-MYPT1(1--314)

   Depositor

   Mathieu Bollen

   Article

   Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

   Insert

   LgBiT-MYPT1(1-314)KAKA-Flag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LgBiT-ST

   Plasmid

   #207633

   Purpose

   A plasmid encoding LgBiT fused to SpyTag

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   LgBiT-SpyTag
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac-His-Tipin

   Plasmid

   #22891

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Tipin (TIPIN Human)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pEF_dCas9

   Plasmid

   #68416

   Purpose

   Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUBC_V5_MS2-mCherry

   Plasmid

   #68420

   Purpose

   Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   MS2
   

   Use

   Synthetic Biology

   Tags

   V5 and mCherry

   Expression

   Mammalian

   Mutation

   MS2: V75E/A81G

   Promoter

   human UbC

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hNox5

   Plasmid

   #69354

   Purpose

   expresses human Nox5 in mammalian cells

   Depositor

   Karl-Heinz Krause

   Article

   Banfi et al J Biol Chem. 2001 Oct 5;276(40):37594-60

   Insert

   hNox5 (NOX5 Human)
   

   Use

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-MP2

    Plasmid

    #36097

    Purpose

    3rd generation lentiviral plasmid

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP_sfCherry2(11)_H2B

    Plasmid

    #82605

    Purpose

    Expresses sfCherry2(11) tagged H2B in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Gly12 to Ala in sfCherry11+

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP_TOMM20_sfCherry2(11)

    Plasmid

    #83033

    Purpose

    Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    TOMM20_sfCherry+11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Tat101(AD8)-Flag

    Plasmid

    #115777

    Purpose

    pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

    Depositor

    Damian Purcell

    Article

    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

    Insert

    HIV-1 Tat protein (AD8)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-SC35-cMyc

    Plasmid

    #44721

    Depositor

    Kathleen Scotto

    Article

    Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

    Insert

    SC35 (SRSF2 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMVTO-GFP-ECL-3TM-tevs-KKMP V3

    Plasmid

    #179640

    Purpose

    TEVP-inducible membrane-bound GFP RELEASE construct

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    Membrane-bound GFP RELEASE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-dCasRx-ccdB

    Plasmid

    #221003

    Purpose

    Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-HxB.S

    Plasmid

    #65415

    Purpose

    contains complete cDNA for TNC small splice variant flanked by NotI-KpnI

    Depositor

    Harold Erickson

    Article

    Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

    Insert

    tenascin C small splice variant (TNC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC1802_pmax-RBFOX1N-dCasRx-C

    Plasmid

    #118635

    Purpose

    Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    RBFOX1N-dCasRx-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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