Skip to main content
Addgene
Showing: 801 - 810 of 1308 results

  1. pUPD2 PhyB (GB2721)

    Plasmid
    #160603
    Purpose
    chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.
    Depositor
    Diego Orzaez
    Insert
    PhyB
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160603-plasmid-map-sequence-id-322052

    Enlarge

  2. INCTbiosyn-p35S-GFP(rc)phiC31

    Plasmid
    #127527
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127527-plasmid-map-sequence-id-250842

    Enlarge

  3. INCTbiosyn-p35S-GFP(rc)Bxb1

    Plasmid
    #127528
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127528-plasmid-map-sequence-id-250843

    Enlarge

  4. INCTbiosyn-p35S(rc)2_4_5-GFP

    Plasmid
    #127520
    Purpose
    Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127520-plasmid-map-sequence-id-250826

    Enlarge

  5. pRL_CT_GI_del1

    Plasmid
    #98744
    Purpose
    For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    phytochrome interacting factor 3 (PIF3 Mustard Weed)
    phytochrome B (PHYB Mustard Weed)
    Use
    Synthetic Biology
    Tags
    SV40-NLS, VP64-AD, and synTALE-DBD
    Expression
    Yeast
    Mutation
    mutated nucleotide 576 C to A, deleted amino acid…
    Promoter
    FBA1 and TDH3
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98744-plasmid-map-sequence-id-189858

    Enlarge

  6. pVZ_PcoaT_SQE1

    Plasmid
    #103860
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    squalene epoxidase 1 (XF1 Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted gene is codon optimized. 2nd amino acid …
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    103860-plasmid-map-sequence-id-202305

    Enlarge

  8. pVZ_PcoaT_SQE1_MRN1

    Plasmid
    #103858
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and MRN1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    marneral synthase 1 (MRN1 Mustard Weed)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized2nd amino acid …
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    103858-plasmid-map-sequence-id-202616

    Enlarge

Showing: 801 - 810 of 1308 results