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Addgene

We narrowed to 4,410 results for: Ado;

Showing: 2401 - 2430 of 4410 results
  1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh1-guide3

    Plasmid
    #125402
    Purpose
    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs182222907 (C2CD4A/B GWAS locus)
    Depositor
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR C2CD4AB-enh3-guide3

    Plasmid
    #125410
    Purpose
    CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs17205526 (C2CD4A/B GWAS locus)
    Depositor
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118163
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pNLF1-N_PWP1:1-501

    Plasmid
    #210974
    Purpose
    Mammalian expression of full-length human PWP1. N-terminal NanoLuc luciferase tag.
    Depositor
    Insert
    PWP1:M1-S501 (PWP1 Human)
    Use
    Tags
    NanoLuc luciferase
    Expression
    Mammalian
    Mutation
    E165D F448L G449R not annotated variants
    Promoter
    CMV
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pFBOH-MHL_SHKBP1:182-585

    Plasmid
    #210935
    Purpose
    Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Insert
    SHKBP1:T182-L585 (SHKBP1 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    Q507L natural variant (VAR_036714)
    Promoter
    Polyhedrin
    Available since
    March 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pFBOH-MHL_WDR13:142-485

    Plasmid
    #210924
    Purpose
    Insect expression of human WDR13 fragment A142 to K485. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Insert
    WDR13:A142-K485 (WDR13 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    H325R natural variant (VAR_060284)
    Promoter
    Polyhedrin
    Available since
    March 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. pFBOH-MHL_WDR12:90-417

    Plasmid
    #210903
    Purpose
    Insect expression of human WDR12 fragment T90 to T417. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Insert
    WDR12:T90-T417 (WDR12 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    introduced mutations F99A K131A R319S
    Promoter
    Polyhedrin
    Available since
    March 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 2401 - 2430 of 4410 results