Addgene

We narrowed to 3,557 results for: eed

Showing: 1601 - 1620 of 3557 results

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pCMV-myc3-Mdm2 p60

Plasmid

#52059

Purpose

expression of truncated Mdm2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Mdm2 p60 (truncated) (MDM2 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

p60 (truncated at D367, DVDP motif)

Promoter

CMV

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pEX-CMV-SP-YFP-STM1(24-685) EF hand 3 mut (A79K, N80D, D82G)

Plasmid

#18865

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal interaction molecule 1 (STIM1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

3 mutations (A79K, N80D, D82G) in the EF hand of …

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-BRCA1 3'UTR

Plasmid

#29477

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

BRCA1 3'UTR (BRCA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
MSCV-shCasp2-IRES-GFP

Plasmid

#52061

Purpose

stable knockdown of Caspase-2 in mammalian cells

Depositor

Tyler Jacks, Trudy Oliver

Article

Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

Insert

Caspase-2 shRNA (CASP2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)

Plasmid

#20174

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D) (STIM2 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

3 mutations (K83A,D84N,G86D) in the EF hand of ST…

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pGL3-321/+120 mut1&2

Plasmid

#35546

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

T to G at position -52 and T to G at position -96…

Promoter

none

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pAP1892-1

Plasmid

#105247

Purpose

human GFP11 with tagRFP 33bp downstream in Lamin A/C

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

Insertion of GFP11 at cut tagRFP 33bp downstream in Lamin A/C (LMNA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAP1716-1

Plasmid

#105245

Purpose

human eGFP with homology arms for Lamin A/C

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with homology arms for Lamin A/C (LMNA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-Luc-KLF4-K1S

Plasmid

#62979

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Expression

Mutation

Fragment 1-446 of KLF4

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pET45b-tsr(B/X) (6X-His-Dm-Cofilin)

Plasmid

#128278

Purpose

Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial cell culture

Depositor

Anna Sokac

Article

Figard et al Cell Rep. 2019 Mar 26;26(13):3493-3501.e

Insert

twinstar (tsr) (tsr Fly)

Use

Tags

6X Histidine tag

Expression

Bacterial

Mutation

Met1 changed to Pro1; Cys77 silent basepair mutat…

Promoter

T7

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-321/+120 Ebox mut1

Plasmid

#35544

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

T to G at position -96 in promoter

Promoter

none

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-321/+120 Ebox mut2

Plasmid

#35545

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Expression

Mutation

T to G at position -52 in promoter

Promoter

none

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK2-AURKB 3'UTR

Plasmid

#29475

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

AURKB 3"UTR (AURKB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
CMV-FFluc-GPD1L-3'UTR Full mut

Plasmid

#32489

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated in the entire miR-210 binding region

Promoter

CMV

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only
ER-mit RspA-splitFAST (lentiviral plasmid)

Plasmid

#233604

Purpose

Expression of ER-mit RspA-splitFAST by a single lentiviral plasmid encoding the two fragments

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-splitFAST

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV/SV40

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-RepoMan-S893D

Plasmid

#204995

Purpose

Expresses shRNA resistant GFP-RepoMan S893D Mutant

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 893 to Aspartic acid

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-Aurora B KD

Plasmid

#184043

Purpose

Expresses H2B-GFP fused to Aurora B kinase dead

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

AURKB (AURKB Human)

Use

Tags

EGFP and H2B

Expression

Mammalian

Mutation

Changed Lysine 65 to Arginine

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-Repo-Man-1-890

Plasmid

#184272

Purpose

Expresses C-terminally truncated GFP-RepoMan

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

truncated Repo-Man after aminoacid 893

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-VIM270-pCM

Plasmid

#215869

Purpose

This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

Depositor

Kumiko Ui-Tei

Article

Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

Insert

target site which is completely complementary to passanger strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

Plasmid

#216378

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

HA signal sequence-FLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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