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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,175 results for: tef

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Showing: 1601 - 1620 of 3175 results

  1. pCFEX1-A2aGH

    Plasmid
    #160546
    Purpose
    pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Publication
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    Expression
    Yeast
    Mutation
    N/A
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160546-plasmid-map-sequence-id-317845

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  3. pCRISPEY-GAL-Kan-ADE2

    Plasmid
    #232105
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Publication
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232105-plasmid-map-sequence-id-462309

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  5. pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)

    Plasmid
    #44084
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44084-plasmid-map-sequence-id-61690

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  6. pZC10 (pFA6a-PSc-6xGly-V5-His3MX)

    Plasmid
    #44067
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44067-plasmid-map-sequence-id-61693

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  7. pZC05 (pFA6a-TEV-6xGly-PrtA-KanMX)

    Plasmid
    #44096
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44096-plasmid-map-sequence-id-61688

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  8. pTF260 (pFA6a-PSc-6xGly-KanMX)

    Plasmid
    #44086
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, (XhoI-KpnI-XbaI)-containing spacer,…
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44086-plasmid-map-sequence-id-61663

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  9. pTF274 (pFA6a-PSc-6xGly-3xFlag-HphMX)

    Plasmid
    #44077
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44077-plasmid-map-sequence-id-61678

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  10. pZC12 (pFA6a-PSc-6xGly-V5-KanMX)

    Plasmid
    #44091
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44091-plasmid-map-sequence-id-61695

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  15. pTXB3-Flag PLK1

    Plasmid
    #68271
    Purpose
    bacterial expression of Plk1
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  17. pCas9-EcRT-GAL-Kan

    Plasmid
    #232092
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Publication
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232092-plasmid-map-sequence-id-459849

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  20. pBY011-AMN1ko

    Plasmid
    #183099
    Purpose
    S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.
    Depositor
    Gabor Balazsi
    Publication
    Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:
    Insert
    AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
    Tags
    KanMX
    Expression
    Bacterial and Yeast
    Mutation
    only includes external sequences (504 bp each) as…
    Promoter
    URA3, TEF1
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183099-plasmid-map-sequence-id-361230

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Showing: 1601 - 1620 of 3175 results