We narrowed to 4,027 results for: gem

1. pEGFP-PH1(WT)

   Plasmid

   #86599

   Purpose

   Expresses eGFP-tagged PH(WT) domain of human Akt1

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 PH domain (AKT1 Human)
   

   Expression

   Mammalian

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTagBFP-Akt1

   Plasmid

   #86594

   Purpose

   Expresses TagBFP-tagged human Akt1(WT)

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-sgArid1a-sg3

   Plasmid

   #192169

   Purpose

   Lenti-sgArid1a-sg3

   Depositor

   Sidi Chen

   Article

   Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

   Type

   Empty backbone

   Use

   Lentiviral

   Mutation

   NA

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-sgKmt2d-sg1

   Plasmid

   #192166

   Purpose

   Lenti-sgKmt2d-sg1

   Depositor

   Sidi Chen

   Article

   Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

   Type

   Empty backbone

   Use

   Lentiviral

   Mutation

   NA

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fuw CBX8 dCD

   Plasmid

   #121117

   Purpose

   conditional expression

   Depositor

   Emily Dykhuizen

   Article

   Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

   Insert

   CBX8 72-389 (CBX8 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   contains amino acids 77-389

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fuw CBX8 3xRA

   Plasmid

   #121115

   Purpose

   conditional expression

   Depositor

   Emily Dykhuizen

   Article

   Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

   Insert

   CBX8 (R19A, R20A, R22A) (CBX8 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   R19A, R20A, R22A

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMMK ENTR 2

   Plasmid

   #206261

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Type

   Empty backbone

   Use

   Multimate/gateway entr 2

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. VAMP2-12aa-mCit

   Plasmid

   #53237

   Purpose

   Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Tags

   12aa-linker and mCitrine

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

9. M13cp-dg1 hp

   Plasmid

   #218093

   Purpose

   Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pI

   Depositor

   Kevin Esvelt

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_FLAG-NFL(WT)

    Plasmid

    #182659

    Purpose

    Expresses wild-type mouse neurofilament light chain with an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    wild type mouse neurofilament light chain (Nefl Mouse)
    

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMMK ENTR 1

    Plasmid

    #206260

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Multimate/gateway entr 1

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMMK ENTR 3

    Plasmid

    #206262

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Multimate/gateway entr 3

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMMK ENTR 4

    Plasmid

    #206263

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Multimate/gateway entr 4

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFBD-Akt1(WT)

    Plasmid

    #86561

    Purpose

    Expresses human full-length Akt1(WT)

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVPS13D-I-EGFP

    Plasmid

    #176464

    Purpose

    express internally GFP-tagged VPS13D in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    EYFP is internally inserted

    Promoter

    CMV

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid

    #52495

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-X1-Neo-NDUFB2

    Plasmid

    #139841

    Purpose

    Lentiviral expression of human NDUFB2

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    NDUFB2 (NDUFB2 Human)
    

    Use

    Lentiviral

    Available Since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFKBPmCherry-Akt2(K14A)

    Plasmid

    #86597

    Purpose

    Expresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutant

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    FKBB-mCherry-Akt2(K14A) (AKT2 )
    

    Expression

    Mammalian

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-Akt2(K14A/R25E)

    Plasmid

    #86595

    Purpose

    Expresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutant

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt2(K14A/R25E) (AKT2 )
    

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only